This investigation was led to recognize FecX^H and FecX^G changes in Karadi sheep. Right now Karadi sheep (4 single and 11 twining) were researched for the nearness of the FecX^H and FecX^G transformation utilizing PCR-RFLP. PCR items 240 and 141 bp was processed utilizing SpeI and HinfI limitation compound to identify FecX^H and FecX^G point Mutations, separately. The FecX^H and FecX^G DNA test demonstrated that there were no bearers for the FecX^H and FecX^G transformations in the chose Karadi sheep. These outcomes recommend that fruitfulness of this breed isn't connected to similar changes. In this way, it is important to look for different transformations or fertility qualities so as to create marker help choice methods and study the productive system of the Karadi breed.