The present study aimed to investigate the effect of dietary graded levels of Aqua Superzyme, Garlen Allicin, and Diamond V (Original XPC) on feed utilization by Nile tilapia fish for 16 weeks. Dietary inclusion of the tested pre-and probiotics improved significantly (P≤0.05) all criteria measured including feed utilization parameters, digestibility, and energy retention. The significantly (P≤0.05) best results of feed utilization were calculated for the treatments Aqua Superzyme at 0.01%, Garlen Allicin at 0.01%, and Diamond V-Original XPC at 0.5% of the diet. Yet, the comparison among additives and their levels shows that the overall significantly (P ≤ 0.05) best treatment was Garlen Allicin at 0.01% of the diet. Concerning the digestibility, the significantly (P ≤ 0.05) best level within each treatment was also Aqua Superzyme at 0.01%, Garlen Allicin at 0.01%, and Diamond V-Original XPC at 0.5% of the diet, respectively. The CF followed by EE were more positively affected than the other nutrients digestibility. The comparison among additives and their levels on ADC clears that the significantly (P ≤ 0.05) best treatment and level was Aqua Superzyme at 0.01% of the diet for ADC of all nutrients, comparing with the other additives and levels. Aqua Superzyme at 0.02%, Garlen Allicin at 0.01%, and Diamond V-Original XPC at 0.5% of the diet, respectively were the best in energy retention (in an ascending order) among their corresponding levels. The comparison among treatments revealed that best energy retention was realized by the treatment of Diamond V-Original XPC at 0.5% of the diet. Generally, it is recommended to add  Garlen Allicin at 0.01% of the tilapia diet to improve its feed and nutrients utilization.