This study was conducted to evaluate the effect of feeding different levels of DDGS in the diets of tilapia fingerlings on growth performance, feed utilization, chemical composition of the whole fish body, blood hematological and economic efficiency. Therefore, six graded levels of DDGS (0, 4, 8, 12, 16 and 20%) were used insteated of fish meal and yellow corn protein in sex tested diets approximately isonitrogenous and isocaloric. Fish were stocked in a rearing plastic tank for two weeks adaptation period, then it were stocked at the rate of 5 fish/glass aquarium with initial weight of 6.0 ± 0.14 g/fish. During the experimental period (72 days) the fish were fed the tested diets at the rate of 6% of the total biomass for six days per week. The obtained results showed that : There were significant (P ≥ 0.05) effect of DDGS levels on all traits of growth performance and feed utilization parameters of Nile tilapia fed different levels of DDGS. All growth performance parameters were increased significantly (P ≤ 0.05) with increasing the replacement levels of DDGS until 16% of DDGS, then, decreased significantly with increasing the replacement level of DDGS. The highest values of growth performance parameters were found in replacement levels 16 % of DDGS. The best values of economic efficiency recorded with the level 12% and 16% of DDGS, respectively. It may be concluded  that replacing fish meal and yellow corn by DDGS until 16% in the diets of tilapia had no any adverse effect on most of growth parameters, feed utilization, chemical composition of fish body, hematological blood parameters and economic efficiency of tilapia fingerlings.