The objective of the present study was to determine the effects of dietary inclusion of different levels of pawpaw seeds powder (PSP) at different periods on the growth performance, survival rate, feed utilization and carcass composition of fry and fingerlings of Nile tilapia (Oreochromis niloticus). The experiment was divided into two periods; the first was feeding period of Nile tilapia fry with PSP for 71 days; and, the second was rearing period where the Nile tilapia fingerlings fed control diet (0 g/kg diet from PSP) for 84 days. Apparently-healthy 1200 Nile tilapia fry of one day old with an average initial body weight 0.012 g/fry were randomly distributed to 20 treatments. Each treatment was referred to three aquaria. The PSP was added to the basal diet at levels of 0, 2, 4, 6 and 8 g/kg diet for 15, 30, 45 and 60 days, different periods. The results of the first period showed no significant (P ≥ 0.05) differences in all growth performance parameters and feed conversion ratio (FCR) during all periods (15, 30, 45 and 60 day); however, different levels of PSP showed significant (P ≤ 0.01) improvement in all growth performance, survival (intervals SR and final SR) and feed utilization (FI and FCR) by increasing level of PSP till 6 g PSP/ kg diet, then significantly decrease (P ≤ 0.01) till 8 g PSP / kg diet. In case of the second period, all growth performance parameters and feed utilization (FI and FCR) gradually (P ≤ 0.01) improved with increasing feeding periods until 45 day, and then decreased with increasing feeding period (60 day). The interaction between levels of PSP and different periods of treatment indicated that 6 g PSP/kg diet for 45 day had the best (P ≤ 0.01) values of FW, TWG and ADG compared with other treatments. Thus, the results of this study lead to useful and safety recommended use of 6 g PSP /kg diet for 45 day, to be economic and efficient for fish farms.