The main objective of this study was to evaluate aflatoxin (AFs) removal ability of probiotic bacteria.  Two hundred and seventy one-day-old (Ross 308) broiler chicks were randomly distributed among nine groups. The dietary treatments were: 1 control; 2&3 artificially contaminated diets with 0.5&1mg of AFs, respectively; 4&5 received oral gavages anaerobic caecal culture (probiotic). 6, 7, 8&9 AFs-contaminated diets plus probiotic feed additive (AFS-PB). The criteria of evaluation included body weight gain (BWG), Feed consumption (FC), and feed conversion ratio (FCR). The toxic effects of aflatoxins were apparently in groups received AFs contaminated diet which showed significantly lower  BWG and FC and impaired FCR compared with control diet. Such effects overcome by adding probiotic bacteria to aflatoxin treated groups. On the other hand, AFs treated group showed significant increased in serum liver function enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST) ), urea and creatinine levels. The intakes of probiotic bacteria significantly alleviated the elevation of ALT, AST, urea and creatinine levels. The results indicated the effect of probiotic as a potential protective agent against aflatoxin toxicity to decrease the risk of occurrence of liver and kidney dysfunction and occurrence of cancer and protect against both membrane and DNA damage.