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70514

IMPACT OF REPLACING EGG YOLK WITH LECITHIN ON SPERM CHARACTERISTICS, BACTERIAL COUNT AND FERTILIZING ABILITY OF CRYOPRESERVED BUFFALO SEMEN.

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Last updated: 04 Jan 2025

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Abstract

Soy bean lecithin has been used to replace egg yolk in the semen extender. The aim of this study was to evaluate the effect of replacing egg yolk by soy-lecithin in tris extender on freezability and fertilizing capacity of buffalo semen. Semen from five buffalo bulls was frozen in tris extender containing 0.5, 1.0 and 2% soy-lecithin (treatments) or 15% egg yolk (control). Percentage of motility, livability and intact acrosome spermatozoa and also bacterial count was assessed in diluted, post-equilibrated and post-thawed semen. Results revealed insignificant differences in percentage of sperm motility in diluted semen. However, percentages of sperm motility, livability and intact acrosome spermatozoa increased (P<0.05) and bacterial count decreased (P<0.05) in post-equilibrated and post-thawed semen extended with 0.5% soy-lecithin. These results reflected in the highest conception rate (66.7%) of buffalo cows inseminated with semen containing 05% soy-lecithin compared with 53.3% in semen containing egg yolk (control).
In conclusion, egg yolk could be replaced by soy-lecithin in tris-based extender at a level of 0.5% to increase fertilizing capacity of cryopreserved buffalo semen. 
 

DOI

10.21608/jappmu.2014.70514

Keywords

Buffalo, frozen semen, sperm, bacterial count, fertility

Authors

First Name

M.

Last Name

El-Sherbieny

MiddleName

A.

Affiliation

Animal Production Research Institute, Agricultural Research Center, Egypt.

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Volume

5

Article Issue

6

Related Issue

10368

Issue Date

2014-06-01

Receive Date

2014-06-08

Publish Date

2014-06-01

Page Start

353

Page End

364

Print ISSN

2090-3642

Online ISSN

2090-3723

Article File

JAPPMU_Volume 5_Issue 6_Pages 353-364.pdf

PDF . 441.9kB

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https://jappmu.journals.ekb.eg/article_70514.html

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https://jappmu.journals.ekb.eg/service?article_code=70514

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Original Article

Type Code

876

Publication Type

Journal

Publication Title

Journal of Animal and Poultry Production

Publication Link

https://jappmu.journals.ekb.eg/

MainTitle

IMPACT OF REPLACING EGG YOLK WITH LECITHIN ON SPERM CHARACTERISTICS, BACTERIAL COUNT AND FERTILIZING ABILITY OF CRYOPRESERVED BUFFALO SEMEN.

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Article

Created At

22 Jan 2023