This study aimed to investigate effect of green tea level as a dietary additive on semen quality and testosterone concentration of adult APRI line rabbit bucks. A total of 12 adult bucks were allotted into 4 equal groups fed complete feed diet (17.4% CP and 2257 Kcal metabolizable energy on dry matter basis), without additive (T1) or supplemented with 2, 4 and 6 g green tea/kg diet (T2, T3 and T4 respectively). The experimental period was 13 weeks, 4 weeks as a preliminary period and 9 weeks as main period of semen collection. Semen was evaluated for volume (SV), pH value, and percentages of motility (SMP), livability (SLP) and abnormality (SAP) of spermatozoa as well as sperm cell concentration (SCC). Sperm count as total output (TSO) and total motile (TMO), total live (TLO) and total normal (TNO) was calculated. Testosterone concentration in blood plasma was determined pre-treatment, and mid and end of the collection period. Results showed that bucks in T4 treated with 6 g green tea/kg diet showed the highest (P<0.05) SV, pH value, SMP, SCC, TSO, TMO, TLO, TNO and the lowest (P<0.05) SAP. However, SLP and testosterone concentration were not affected by treatment. In conclusion, green tea demonstrates significant improvement in the antioxidant status, as shown by the increased antioxidant enzyme activity and GSH levels. Green tea could serve as a supportive treatment in the nutritional management to improve semen quality of rabbit bucks, particularly at a level of 6 g/kg diet. Keywords: Rabbit, green tea, semen, sperm output, testosterone. This study aimed to investigate effect of green tea level as a dietary additive on semen quality and testosterone concentration of adult APRI line rabbit bucks. A total of 12 adult bucks were allotted into 4 equal groups fed complete feed diet (17.4% CP and 2257 Kcal metabolizable energy on dry matter basis), without additive (T1) or supplemented with 2, 4 and 6 g green tea/kg diet (T2, T3 and T4 respectively). The experimental period was 13 weeks, 4 weeks as a preliminary period and 9 weeks as main period of semen collection. Semen was evaluated for volume (SV), pH value, and percentages of motility (SMP), livability (SLP) and abnormality (SAP) of spermatozoa as well as sperm cell concentration (SCC). Sperm count as total output (TSO) and total motile (TMO), total live (TLO) and total normal (TNO) was calculated. Testosterone concentration in blood plasma was determined pre-treatment, and mid and end of the collection period. Results showed that bucks in T4 treated with 6 g green tea/kg diet showed the highest (P<0.05) SV, pH value, SMP, SCC, TSO, TMO, TLO, TNO and the lowest (P<0.05) SAP. However, SLP and testosterone concentration were not affected by treatment. In conclusion, green tea demonstrates significant improvement in the antioxidant status, as shown by the increased antioxidant enzyme activity and GSH levels. Green tea could serve as a supportive treatment in the nutritional management to improve semen quality of rabbit bucks, particularly at a level of 6 g/kg diet.