This work aimed to analyze the result of in-ovo injection at the 14^th day of hatching period on hatchability, embryonic mortality (%) and subsequent growth performance additionally physiological status for hatched chicks. A 540 hatching Sinai eggs were collected, weighted (52 ± 1 g), distributed into 6 groups and incubated. At the 14^th day of incubation, all eggs were candled then total fertile eggs of each group spliced into equal 3 replicates. Experimental groups were organized as follow: negative control (NC) without injection while the other groups were injected with sterile distilled water alone as a positive control (PC), spirulina (SP) with 2.5 and 5.0 mg/egg, canthaxanthin (CX) with 0.025 and 0.050 mg/egg, respectively. Results indicated that the highest value of hatchability (%) of fertile eggs was achieved by injected eggs with 0.050 mg CX/egg followed by in-ovo injection with 2.5 mg SP/egg in comparison with the PC and NC groups. In-ovo injection with 0.050 mg CX/egg resulted in a heavier chick body weight at 8 days after hatch and relative heart weight than other treatments and the control groups. Chicks produced from the eggs injected with 0.050 mg CX / egg recorded a significant improvement in BW at 28 days of age and subsequent BWG than other injected groups and NC during 0-28 days of age. The highest value of the survival ratio percentage was recorded for chicks produced from injected eggs with 2.5 mg SP/ egg. Total protein, globulin and HDL recorded higher (P≤0.01) values in chick's serum by injected hatching eggs with 0.025 and 0.050 mg CX/egg in comparison with NC group, however, total cholesterol and LDL recorded the lower value. It was concluded that, in-ovo injection Sinai hen's eggs with spirulina and canthaxanthin could be used to improve hatchability and decrease embryonic mortality (%) and enhance subsequent growth performance additionally physiological situation of hatched chicks.