Aflatoxin contamination of animal diet is still a major problem for breeders since it hasadverse effects on animal health and productivity. This study was carried out to investigate the effect of using coumarin and/or chlorophyll in rat diet against aflatoxicosis. Fifty four rats were randomly assigned into 7 groups ''6 rats each'' and group 5 ''12 rats''. Group 1 was negative control. Group 2 received water with coumarin 0.5%. Group 3 received water with chlorophyll 0.5%. Group 4 received water with coumarin 0.5% and chlorophyll 0.5%. Group 5-8 received basal diet with aflatoxin B₁ 1000 ppb. Group 6-8 were administered similar treatments as groups 2-4. The experiment ended after 8 weeks. Water and feed consumption were measured and feed conversion rate were calculated. Random glucose level, total lipid, total cholesterol, triglyceride, total protein,  ALT , AST, creatinine  and urea were determined in serum. Histopathological examination of liver, kidney and pancreas was performed. The conversion rate was significantly low in group 5 compared to groups 6-8. The serum glucose, cholesterol, AST and ALT were elevated in group 5 compared to groups 6-8. The liver lesions observed in group 5 represented in vacuolation and necrosis were alleviated in groups 6-8. The necrosis and inflammatory cells infiltration in pancreas of group 5 were absent in groups 6-8. In conclusion, the coumarin and or chlorophyll  possessed a hepatoprotective effect against aflatoxincosis.