The aim of the present study was to define the effect of different extender components (as conventional and non-conventional extenders) on rams' semen quality during storage at 5ºC for up to 6 days. Fertility rates of the extended ram spermatozoa stored at 5ºC for up one day were also studied. Four Rahmani rams were used to collect pooled ejaculates by artificial vagina. Semen was collected, evaluated and extended with five extenders included conventional and non- conventional extender ingredients. The conventional extenders were sodium citrate (E1), Tris (E2) and skimmed milk powder (E3) - based glucose- egg yolk. While, non-conventional extenders were salt of sodium chloride 0.9% (E4) and saline solution 0.9% intravenous infusion (E5) based glucose-egg yolk. The final extension rate was 1semen : 6 extender. The extended semen with different extenders media (E1, E2, E3, E4 and E5) were cooled to 5ºC and stored at this temperature for 6 days. After storage time (0, 1, 2, 3, 4, 5 and 6 days), the percentage of sperm motility (SPM), recovery sperm motility (RSM) and positive osmotic resistant (POR) were recorded. Also, fertility rate was carried out with liquid semen stored at 5ºC for up to one day using the best conventional and non-conventional extenders. Thus, twenty-two ewes were used to investigate fertility rate. Ewes were divided into two groups (11/group), the1^st and 2^nd groups were artificially inseminated with conventional (E2) or non-conventional (E5) extenders which obtained better semen quality than other extenders, respectively. The results indicated that, the cooling extended ram semen with each of E1, E2 or E5 extenders was significantly (P<0.05) higher the percentage of SPM, RSM and POR than those extended with E3 and E4 extenders during storage at 5ºC for up to 6 days. However, the cooling ram semen with E2 and E5 showed non-significantly parameters during cooled at 5ºC till 6 days. The statistical analysis of the data revealed a significant positive correlation between extender types and SPM, RSM and POR of ram spermatozoa during storage at 5ºC until 6 days. Surprisingly, fertility rate of ewes artificially inseminated with ram semen preserved at 5ºC for one day of storage performed to assess higher conception rate (84.62%) with E5 ( as non-conventional extender) than conception rate (78.57%) with E2 (as conventional extender). Moreover, the superior litter size recorded 1.00 with ram semen extended with E5 compared to 0.86 with E2 extenders. Also, sex ratio of born lambs displayed female lambs 66.67 and 33.33% of male lambs with semen extended with E2 extender. Ram semen extended with E5 achieved female lambs 69.23% and male lambs 30.77%. It is therefore concluded that saline solution (0.9%) intravenous infusion (E5) based glucose-egg yolk intended to keep goodness fertility rate and litter size after AI of cooled semen at 5ºC.