The present study was investigated the effects of Ethylenediaminet etraacetic acid(EDTA) contaminated food manipulation on the fertility of male mice the investigtion based on evaluation of physical semencharacteristics, andmorphometrical andhistopathological studies. ofForty healthy adult male Swiss Albino mice have been divided into four groups. The first group (the control) received only standard diet while the second to the fourth groups were supplemented daily with contaminated food with different EDTA concentrations, 0.5, 1.5 and 5 g/kg of food. The samples were collected after two experimental periods (four and eight weeks). Results showed that the EDTA manipulated groups exhibited significantlydecrease of sperm parameters and testosterone concentration. Morphometric study of testis in different EDTA manipulated groups showed a significant decrease of primary spermatocytes and fertile seminiferous tubules in count as compared to control group. However, histological results showed decrement of spermatogeniclineage;declined spermatozoa formation and several necrotic areas. Also, some Sertoli cells and interstitial cells were degenerated. In addition, several blood vessels revealed dilation and congestion in between the interstitial cells. All the adverse alteration in the present results was proportional with the dose of EDTA manipulate on of mice and with the experimental period expansion. In conclusion, the adverse effects of EDTA as a chelating agent induced obviousinhibition of spermatogenic lineage and degeneration of interstitial cells leading to decrease the fertility of male mice.