This study was designed to examine the effect of the age of rabbit and addition of serum at different reproductive status (pre-, at and post-mating doe serum) in maturation medium (TCM-199) as compared as hormonal addition (10 IU eCG and 20 IU hCG/ml) on in vitro maturation of rabbit oocytes. Results show that frequency distribution of oocytes at MII stage was higher (P<0.05) for oocytes recovered from old (59.58%) than young (51.69%) rabbits. Frequency distribution of oocytes at MI stage showed an opposite trend, being significantly (P<0.05) lower for oocytes recovered from old (9.58%) than the small (14.89%). Frequency distribution of oocytes at MII was the highest (62.11%, P<0.05) for oocytes in vitro maturated in maturation medium supplemented with post-mating serum, but did not differ significantly from those in vitro matured in media supplemented with hormones (57.54%) or pre-mating serum (55.87%), while oocytes matured with mating serum showed significantly the lowest values (46.78%). Frequency distribution of oocytes at MI stage showed an opposite trend to that at MII. The interaction between age of oocyte donors and type of serum was not significant on frequency distribution of oocytes at all maturation stages. Such effect was reflected in higher maturation rat (oocyte at MII) for oocytes matured with Post-mating serum for young or old doners, but in vitro maturation rate was the highest (67.5%) for oocytes recovered from older doners and in vitro matured in medium supplemented with post-mating serum. Results of the current study revealed that the highest percentage of oocytes at metaphase-II (matured oocytes) and the lowest percentage of degenerated oocytes were obtained when rabbit oocytes were recovered at older ages and cultured in TCM-199 supplemented with serum from post-mating doe serum or hormones (eCG and hCG).