This study was carried out to investigate the effect of different oils (sunflower, fish and linseed oils) as sources of omega 3 and 6 with or without vitamin E (VE) supplementation, on semen quality and reproductive performance of turkey toms. Thirty six Bronze turkey toms at thirty-six weeks of age were used, birds were divided to equal homogenous three groups (n=12 toms). The 1^st group was fed the basal diet containing 2% sunflower oil (SO, as control), while the 2^nd and 3^rd groups were fed the same diet with 2% fish oil (FO) or linseed oil (LO) instated of SO. All groups were divided into two subgroups (n=6 toms), the 1^st subgroup was fed diet without vitamin E (VE) supplementation (the diet contain 50 mg as alpha-tocopheryl acetate/kg diet from premix, VE), while the 2^nd subgroup was fed diet supplemented with 100 mg VE/kg diet as anti-oxidant (150 mg VE /kg diet as a total content). Results indicated that FO or LO in the diet of turkey toms at a level of 2% may affect slightly weight without any adverse effect on body weight  at 48 wks of age or body weight gain (36-48 wks of age). Dietary supplementation of VE increased (P<0.05) body weight as compared to those fed diet without VE. Semen characteristics of toms were affected (P<0.05) by omega-3 sources and VE supplementation, except for ejaculate volume. Tomes fed 2% FO+150 mg VE/kg diet had higher (P<0.05) percentages of sperm motility (75.66%) and livability (82.61%), followed by those fed the same oil diet without VE (74.70 and 81.50%) and LO +150 mg VE/kg diet (74.24 and 81.38%) as compared to other treatment groups, respectively. All treatments increased (P<0.01) fertilizing ability of toms and hatchability rate of total eggs. Marked differences were noted among dietary treatments in the histological examination of testicular tissue and epididymis of toms. This study concluded that turkey tom diets containing 2% fish oil or linseed oil plus 150 mg VE/kg diet as a total content are needed to obtain the best reproductive performance, semen quality, fertility and hatchability.