Lactoferrin (LF, 80kDa) is secreted by epithelial cells into milk which has the ability to hold iron that gives LF special bioactivities as antipathogenic, immune modulation, anti-oxidant, growth factor and cell proliferation stimulating glycoprotein. The current study aims to investigate the effect of different concentrations of bovine lactoferrin (bLF) on in vitro maturation, in vitro fertilization and embryo development of Egyptian buffalo oocytes. Each of in vitro maturation, in vitro fertilization (IVF) and embryonic development medium was supplemented with bLF at concentrations of zero (control), 0.01, 0.03, 0.05 and 0.1 mg/ml for each trail. Lactoferrin insignificantly enhanced maturation rates compared with control group. The highest rates (p<0.001) of IVF (52.73 and 43.33%) were observed for both 0.03 and 0.05 mg/ml supplementations to IVF medium. All bLF levels increased (p<0.0001) cleavage rate and proportion of embryos at both morula and blastocyst (p<0.05) stages than the control medium with advantage to 0.03 mg/ml concentration along examined developmental stages. The current study revealed that low levels of bovine bLF has a supportive effect (especially 0.03 mg/ml) on each of IVF media and embryo development of the Egyptian buffalo oocytes.