Embryo cryopreservation becomes a pivotal side in the shape of assisted reproductive technologies. It plays an essential role in several cases such as, embryo storing during transportation, embryo preservation for future usesand establishment ofcryobanks for endangered species and rare breeds. The presentexperiment was conducted to evaluate the cryotoleranceofin vitro produced buffalo embryosat blastocyst stage to vitrification process using cryotop. Embryos were produced using conventional in vitro fertilisation technique and had been divided into two categories: non-vitrified group, which was culturedin vitro till hatched blastocyst stage and vitrified group, which was collected at day 7 for cryopreservation. A stepwise vitrification and thawing procedures were performed using Cryotop. Survival rate of embryos was observed within 4 hours of thawing, then the ability to expand and hatch was recorded.The expansion rate was higher in control group compared to vitrified group (91.66 vs. 86.36%, respectively) with no significant differences. In both groups, all expanded blastocysts reached the hatching stage normally.Therefore, our results suggested thatCryotop is aneffective tool for cryopreservation of in vitro produced Egyptian buffalo blastocyst.