Aflatoxins (AFs) contamination in food is a serious problem in the world. Aflatoxin B₁ (AFB₁), produced by Aspergillusflavus,is secondary metabolite, highly toxic and carcinogenic. The reduction of AFs contamination in food and feed products could be achieved by some microorganisms. In this study,Lactobacillus plantarum ATCC 14917, Lactobacillus curvatus ATCC 1136, Bacillus megateirum A.F.10, Bacillus subtitles A.F.12and commercial probiotic were used to inhibit AFB₁ production on yeast extract sucrose medium (YES) and on corn. All bacterial strain inhibitedA. flavusproduction of AFB₁ on YES media and corn. Also, commercial probiotics hadthe ability to inhibit A. flavus growth and its production of AFB₁ on corn. The most effective bacteria wasB. megaterium A.F.10. It inhibited the fungal growth with 12 mm inhibition zone and inhibits AFB₁ production 100% by HPLC on YES media. Determination of AFB₁ production by HPLC on corn showed that B. megaterium inhibitedthe production by 69.81% followed by commercial probiotics (59.62%).Commercial probiotics and B. megaterium had a synergistic effect in inhibition ofAFB₁ production and in vitro digestion of corn.