An experiment was undertaken to study the influence of dietary Aaflatoxin B1 (AFB1) on  layer performance  and the efficacy of some non-nutritive feed additives such as chemical Hydrated sodium calcium Alumino Silicat (HSCAS), prebiotic Mannan-oligosaccharides (MOS) and lactobacillus acidophilus based probiotic (Biotop) to eliminate the adverse effects of AFB1 on productive and reproductive performance, egg quality and some blood constituents of local laying hens. A total number of 150 Dokki-4 hens plus 30 Dokki-4 cocks 28 weeks old, were divided into 5 groups of 3 replicates (10 hen +1 cock) and housed in floor pens .The remaining 15 cocks were also divided into 5 groups of 3 cocks each and reared separately in wire-cages for semen evaluation and fed the same treated diets. Birds were allotted on the following treatments basal diet ( without any additives), basal diet contaminated with 1 ppm aflatoxin B1/kg diet (AFB1-diet), AFB1-diet+5g HSCAS/kg diet, AFB1-diet+1gMos/kg diet and AFB1-diet+1g Biotop/kg diet. All experimental groups were fed on the experimental diets for 8 weeks as treated period, then they were fed free toxin diet for 4 weeks as a recovery period. Criteria of response were productive traits, egg quality, reproductive traits, semen evaluation, plasma biochemical constituents, AFB1 residue of egg yolk, New castle disease virus(NDV) antibodies titer and Protection percentage.The obtained results could be summarized as follows: The three AFB1-detoxifying agents applied significantly ameliorated the deleterious effect of AFB1, on final body weight and body weight gain. Addition with HSCAS, Mos or Biotop increased egg number/hen, average egg weight and egg mass. The best feed conversion ratio was obtained with the control group and dietary supplementation of HSCAS, Mos, or Biotop to AFB1–diet improved (FCR). Feed additives supplementations to AFB1-diet increased yolk weight, shell weight %, shell thickness, yolk color and yolk cholesterol. No significant difference due to treatments among albumen weight %, yolk index %, Haugh unit % and egg shape index %. At the end of the recovery period all egg quality parameters were recovered  in groups fed on AFB1-diets during treated period except those which fed on AFB1-diet alone without feed additives for shell-thickness and yolk cholesterol content, where lower than the control and other treatments. The addition AFB1-detoxifying agents significantly improved semen volume, sperm concentration, motility rate, sperm abnormality and dead sperm. Hens fed diets supplemented with HSCAS, Mos or Biotop recorded higher values of fertility, hatchability and chick weight at hatch and lower values of chick abnormality compared to AFB1-diet. After recovery period alterations caused by AFB1-diet were negated for fertility, hatchability and chick weight at hatch but group previously fed AFB1-diet alone without any additives still recorded significantly higher value of chick abnormality compared with control and other treatments. Addition of  HSCAS, Mos and Biotop significantly reduced the severity of AFB1 effects by increasing plasma total protein, albumin , globulin, Calcium and phosphorus  and decreasing plasma AST,ALT,ALP, Creatinine, cholesterol and uric acid compared to AFB1-diet.  AT the end of recovery period after the withdrawal of the contaminated feed , all groups fed the aflatoxicated diet with HSCAS, Mos or Biotop were recovered for all blood parameters except AST ,ALT ,cholesterol and creatinine. Addition of HSCAS, Mos or Biotop to the aflatoxin contaminated diet significantly ameliorated the harmful effect of aflatoxin on immune response to NDV in all examined samples for hen serm, egg yolk and post-hatch chick. Addition of feed additives (HSCAS, Mos or Biotop) significantly decreased the level of AFB1 residues in egg yolk compared to those fed AFB1-diet alone. After 4 weeks of recovery period, there were no residues in egg yolk. Conclusively, Feeding AFB1 contaminated diet (1 ppm Aflatoxin B1) resulted significant reduction in productive  and reproductive performance of Dokki - 4 laying hens.  Addition of HSCAS, Mos or Biotop to the aflatoxin contaminated diets significantly ameliorated the harmful effect of aflatoxin and can be recommended as antitoxin for detoxification of AFB1in diets of laying hens. Four weeks recovery period was quite good to improve the laying performance.