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54261

EFFECT OF ROYAL JELLY IN HONEY-INCLUDED EGG YOLK BASED EXTENDERS ON MOTILITY, VIABILITY AND FERTILIZING ABILITY OF FROZEN RABBIT SPERMATOZOA

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Last updated: 04 Jan 2025

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Abstract

The present study was designed to investigate the efficacy of Royal Jelly (RJ) addition in honey-included egg yolk and Tris-citric-glucose with dimethysulfoxide (DMSO) for the extension of New Zealand White (NZW) rabbit buck semen conserved frozen. Fifteen sexually mature NZW rabbit bucks, aged 17 months with average body weight of 3.9 kg, were selected for high quality semen. One hundred and forty hybrid nonporous NZW rabbit does, were used for artificial insemination (20 does per each experimental group).
Semen was collected from rabbit bucks, pooled and diluted with 8 different extenders. Control group had Tris-citric-glucose + DMSO + 20% egg yolk, while the other 7 experimental extenders were based on Tris-citric-glucose with DMSO, 2% pure bee honey and 18% egg yolk which were added to diluents (v/v). Royal jelly (RJ) was added in increasing concentrations of 0,10, 20, 30, 40, 50 and 100 mg/6ml diluents  for extenders 1, 2, 3, 4, 5 , 6 and 7, respectively. Evaluations for percentages of progressive motility, dead and abnormal spermatozoa were done immediately after dilution and one month of freezing in liquid nitrogen. Fertilizing ability was evaluated as pregnancy rate and litter size of 20 does inseminated artificially for each extender, which showed ≥ 50% sperm progressive motility after thawing. Extender contained 10 mg RJ/ 6 ml diluent showed the best values for motility, dead and abnormal spermatozoa, also for pregnancy rate and litter size.
Conclusively, the results of the present study proved that the addition of royal jelly in bee honey-included egg yolk based extenders may improve the motility, viability and fertilizing ability of rabbit spermatozoa. However, the effect of RJ on NZW rabbit semen quality and fertility is concentration dependent.
 

DOI

10.21608/ejrs.2013.54261

Keywords

Honey, egg yolk, extenders, motility, fertilizing ability, Rabbit, royal jelly, semen. Royal jelly (RJ) is a secretion product of the cephalic

Authors

First Name

Ahmed

Last Name

El-Sherbiny

MiddleName

-

Affiliation

Animal Production Department., Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

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City

Agriculture, Ain Shams University, Cairo, Egypt

Orcid

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Volume

23

Article Issue

2

Related Issue

7242

Issue Date

2013-07-01

Receive Date

2013-06-03

Publish Date

2013-07-01

Page Start

149

Page End

160

Print ISSN

1110-2594

Online ISSN

2682-3330

Article File

EJRS_Volume 23_Issue 2_Pages 149-160.pdf

PDF . 358.8kB

Link

https://ejrs.journals.ekb.eg/article_54261.html

Detail API

https://ejrs.journals.ekb.eg/service?article_code=54261

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6

Type

Original Article

Type Code

868

Publication Type

Journal

Publication Title

Egyptian Journal of Rabbit Science

Publication Link

https://ejrs.journals.ekb.eg/

MainTitle

EFFECT OF ROYAL JELLY IN HONEY-INCLUDED EGG YOLK BASED EXTENDERS ON MOTILITY, VIABILITY AND FERTILIZING ABILITY OF FROZEN RABBIT SPERMATOZOA

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Type

Article

Created At

22 Jan 2023