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47469

PROGESTERONE AND ESTROGEN SUPPLEMENTATION IN VITRO CULTURE MEDIA IMPROVE MORPHOGENESIS AND DEVELOPMENT GASTRULATION EMBRYOS RABBITS

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Last updated: 04 Jan 2025

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Abstract

The objective of this study was to compare the development competence of in vitro embryo rabbit and gastrula under culture media supplemented with serum pregnancy does rabbit (SPDR). Eighty four rabbit embryos from 20 does at 6d.p.c; divided into two groups. The 1st group culture with stander media TCM 199 (control). The 2nd group culture with TCM 199 supplemented with (10% v/v) pregnancy serum rabbit does with the final concentration of progesterone 9ng/ml and 17β-estradiol 20pg/ml for three days.
Numerically viability rates were improved insignificantly according to SPDR additive to culture media. The increase rate significantly (P˂0.05) in blastocysts dimension (area 74.29%, perimeter 32.80%) and in gastrula dimension (area 82.02%, perimeter 47.24%) at 48h of culture compared with control group. In the other hand, the rate of collapsed embryos elevated in the control group compared with the serum group. Furthermore, the present results were observed that significantly increased (P˂0.05) in gastrulation stages after 8 d.p.c and 9 d.p.c, 65% and 100% of embryos treated with SPDR reached to stage 5 to 7 with strong brachyury expression along the entire primitive streak also in notochordal process .
            Conclusively, overall progesterone and estrogen play an important role in the development and implantation of pre-gastrulation embryos rabbit.
 

DOI

10.21608/ejrs.2014.47469

Keywords

progesterone, embryogenesis, morphogenesis, Gastrulation, in Vitro, Development, Rabbit

Authors

First Name

Ayman

Last Name

Saeed

MiddleName

-

Affiliation

Department of Animal Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt

Email

aymansm66@gmail.com

City

Animal Production Research Institute, Dokki, Giza

Orcid

-

Volume

24

Article Issue

1

Related Issue

6597

Issue Date

2014-01-01

Receive Date

2013-09-05

Publish Date

2014-01-01

Page Start

35

Page End

45

Print ISSN

1110-2594

Online ISSN

2682-3330

Article File

EJRS_Volume 24_Issue 1_Pages 35-45.pdf

PDF . 744.1kB

Link

https://ejrs.journals.ekb.eg/article_47469.html

Detail API

https://ejrs.journals.ekb.eg/service?article_code=47469

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3

Type

Original Article

Type Code

868

Publication Type

Journal

Publication Title

Egyptian Journal of Rabbit Science

Publication Link

https://ejrs.journals.ekb.eg/

MainTitle

PROGESTERONE AND ESTROGEN SUPPLEMENTATION IN VITRO CULTURE MEDIA IMPROVE MORPHOGENESIS AND DEVELOPMENT GASTRULATION EMBRYOS RABBITS

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Article

Created At

22 Jan 2023