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266977

MEAN PERFORMANCE, GENETIC DIVERSITY AND DNA ANALYSIS IN M3 GENERATION OF SOME WHEAT MUTANTS UNDER WATER STRESS

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Last updated: 04 Jan 2025

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Abstract

The field experiments was conducted during the growing season of 2019/2020, to study the mean performance and genetic diversity using RAPD Marker in M3 generation of some wheat mutants with influenced by gamma ray and sodium azaide under water stress conditions, at the Experimental Farm, Faculty of Technology and Development, Zagazig University, Egypt. The all recommended cultural practices for wheat production except irrigation. It was irrigated immediately after sowing the first irrigation were applied after 45 days from sowing and the second irrigation up to the flowering stage. The experimental design was complete blocks design with three replicates. The analysis of variance showed a significant differences among Gimmieza-11 and there M3 mutants for days to heading, spike length, number of spikelets/spike, number of spikes/plant, number of grains/spike, spike grain weight/g, 1000-grain weight/g, grain yield/plant, biological yield/plant and harvest index. The  all mutants from Gemmeiza-11 were give the highest values of  mean performance for grain yield plant, The Sids-12 0.08 sodium azide (SA) had the highest value (45.39) followed by Sids-12 350Gy3 (40.74) and Sids-12 350 Gy1 (39.87) thy were more than control (38.99) for harvest index %. RAPD data analysis showed that, primers applied on wheat genotypes produced 18 bands; 16 of these were polymorphic. Primer OPB-5 gave the highest number of bands (5) and primers O-7, A-20 and OPB-7 had the lowest number (3).The highest percentage of polymorphic bands were produced by primers O-7, A-6 and OPB-5 (100%) and the lowest percentage of polymorphic bands were produced by A-20 and OPB-7 (67%). Conclusively, this study established the importance of gamma rays and sodium azide  mutagenesis increating genetic variation within and among wheat breeding populations. wide phenotypic variation in mutants under each breeding population were identified for improving drought tolerance yield, and yield-related traits.  

DOI

10.21608/jpd.2022.266977

Keywords

mutants, mean performance, Genetic diversity, DNA analysis, Wheat

Authors

First Name

Ragab

Last Name

Abdel-Ghaniy

MiddleName

-

Affiliation

Plant Production, Faculty of Technology and Development, Zagazig University Egypt.

Email

nada__rf@yahoo.com

City

Faculty of Technology and development, Zagazig University Egypt

Orcid

-

First Name

El sayed

Last Name

hasan

MiddleName

-

Affiliation

Plant Production, Faculty of Technology and Development, Zagazig University Egypt

Email

drelsayed11154@gmail.com

City

Faculty of Technology and Development, Zagazig University Egypt

Orcid

-

First Name

Aly

Last Name

Hassan

MiddleName

-

Affiliation

Plant Production, Faculty of Technology and Development, Zagazig University Egypt.

Email

-

City

Faculty of Technology and Development, Zagazig University Egypt.

Orcid

-

First Name

Marwa

Last Name

Ghonaim

MiddleName

-

Affiliation

Cell Research Institute. Agricultural Research Center, Giza., Egypt.

Email

marwa_ghonaim2006@yahoo.com

City

Agricultural Research Center, Giza., Egypt.

Orcid

-

Volume

27

Article Issue

3

Related Issue

28819

Issue Date

2022-07-01

Receive Date

2022-05-17

Publish Date

2022-07-01

Page Start

417

Page End

442

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 27_Issue 3_Pages 417-442.pdf

PDF . 872.3kB

Link

https://jpd.journals.ekb.eg/article_266977.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=266977

Order

5

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

MEAN PERFORMANCE, GENETIC DIVERSITY AND DNA ANALYSIS IN M3 GENERATION OF SOME WHEAT MUTANTS UNDER WATER STRESS

Details

Type

Article

Created At

22 Jan 2023