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221541

MOLECULAR CHARACTERIZATION OF INTERLEUKIN IN CHRONIC HCV.

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Last updated: 22 Jan 2023

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Abstract

A Single nucleotide polymorphism upstream of the interferon-λ3 gene, strongly linked to unprompted and treatment-induced HCV infection clearance. the present study was to assess the role of interleukin- 28 gene polymorphism (rs12979860 CC  and rs8099917 TT) on HCV type 4 Egyptian patients treated with sovaldi. A total of 100 patients with HCV infection was included in this study. the diagnosis was based on elevated alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), plasma albumin level, total bilirubin, direct bilirubin, platelet and HB. Molecular characterization of 2 SNPs (rs 12979860CC  and rs 8099917 TT) in heparinase gene: by molecular techniques (ARMs-PCR & RFLP). IL28B rs8099917 TT genotype is predominant among patients as well as the control followed by GT and the least prevalent was the GG genotype. Regarding iL28B rs8099917 Allele, HCV group was higher in G than the control group, while regarding to T, a control group was higher than HCV group. For polymorphism rs8099917, being higher among patients than controls. Regarding iL28B rs12979860,  HCV group was higher in CT and TT than the control group, while regarding to CC, control group was higher than the HCV group. Regarding iL28B rs12979860 Allele, the HCV group was higher in T than control group, while regarding to C, a control group was higher than HCV group. patients with IL28B rs8099917 TT genotype are associated with a higher probability of spontaneous and treatment-induced HCV clearance than IL28B rs8099917 GT and GG allele carriers. HCV spontaneous clearance was higher in patients with the IL28B rs12979860 CC genotype compared to those with the CT and TT genotypes. Conclusively, treatment with sovaldi can be suggested in patients with IL28B TT genotype and L28B rs12979860 CC genotype to be more effective. Keywords:Interleukin- 28 gene polymorphism, HCV type 4, sovaldi.  

DOI

10.21608/jpd.2022.221541

Keywords

Keywords:Interleukin- 28 gene polymorphism, HCV type 4, Sovaldi

Authors

First Name

Yasmin

Last Name

El-Gohary

MiddleName

-

Affiliation

Molecular Biology Department. Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt

Email

yasminelgohary@outlook.com

City

Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt

Orcid

-

First Name

Khalil

Last Name

Halfawi

MiddleName

-

Affiliation

Molecular Biology Department. Genetic Engineering and Biotechnology Research Institute University of Sadat City, Egypt

Email

-

City

Genetic Engineering and Biotechnology Research Institute University of Sadat City, Egypt

Orcid

-

First Name

Manal

Last Name

Hassan

MiddleName

-

Affiliation

Clinical Biochemistry Depart, Faculty Of Applied Medical Science, 6th October University, Egypt.

Email

-

City

Faculty Of Applied Medical Science, 6th October University, Egypt.

Orcid

-

First Name

Wael

Last Name

Abdelmageed

MiddleName

-

Affiliation

Faculty of Agriculture, Beni-Suef University, Egypt

Email

-

City

Faculty of Agriculture, Beni-Suef University, Egypt

Orcid

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Volume

27

Article Issue

1

Related Issue

28818

Issue Date

2022-01-01

Receive Date

2021-11-09

Publish Date

2022-01-01

Page Start

125

Page End

139

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 27_Issue 1_Pages 125-139.pdf

PDF . 1.1MB

Link

https://jpd.journals.ekb.eg/article_221541.html

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https://jpd.journals.ekb.eg/service?article_code=221541

Order

8

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023