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205853

NATURAL PRODUCTS EFFECT OF TREATMENTS ON GENE TARGETING METABOLISM ON BREAST CANCER

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Last updated: 04 Jan 2025

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Abstract

Breast cancer is among the most common malignant tumors. It is the second leading cause of cancer deaths among women. While mutation in the BRCA1 and BRCA2 genes confer risk of developing breast cancer. Insulin-like growth factors (IGFs) IGF-1 and IGF-2 are associated with the development and progression of breast cancer. Curcumin is a polyphenol natural product isolated from the rhizome of Curcuma longa. For centuries, curcumin has been used in medicinal preparations and as a food colorant. In recent years, Curcumin, has been reported to have anticancer and chemoprevention effects on breast cancer.  Particularly, curcumin has been recognized as an effective anticancer agent that regulates multiple intracellular signaling pathways. This study is designed to investigate the effect of curcumin on gene targeting metabolism on breast cancer. To achieve this aim we conducted the study on MCF-7 cell lines, and evaluate the cytotoxicity of different concentration of curcumin by MTT assay, RNA extracted from MCF-7 cell lines by Qiagen method and reverse transcriptase to cDNA finally quantification of the expression of genes (IGF-1, IGF-2 and BRCA1) by Real time PCR (RT-PCR). The results showed thatdifferent concentration of curcuminmakeinactivation for genes related to breast cancer (IGF1, IGF2 and BRCA1) and can stop the breast cancer pathway. Conclusively, breast cancer is among the most common malignant tumors. It is the second leading cause of cancer mortality among women in the world. Curcumin, an active derivative from turmeric, has been investigated to have anticancer and chemoprevention effects on breast cancer.in this study different concentrations of curcumin influence the expression of gene targeting metabolism on breast cancer and make inactivation to it which may lead to stopping the breast cancer pathway. Our data demonstrated that curcumin could be used as the treatment of breast cancer.         

DOI

10.21608/jpd.2022.205853

Keywords

breast cancer, IGFs genes, BRCA genes, Curcumin

Authors

First Name

Salwa

Last Name

Mohamed

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Affiliation

Molecular Cell Biology & Molecular Biology Department, Genetic Engineering and Biotechnoloy Research Institute,(GEBRI)University of Sadat City, Egypt.

Email

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City

Engineering and Biotechnoloy Research Institute,(GEBRI)University of Sadat City, Egypt.

Orcid

-

First Name

Midaa

Last Name

Ashry

MiddleName

-

Affiliation

Medical Laboratories Department, Faculty of Applied Medical Sciences, October 6 University, Egypt.

Email

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City

Faculty of Applied Medical Sciences, October 6 University, Egypt.

Orcid

-

First Name

Khalil

Last Name

Halfawy

MiddleName

-

Affiliation

Molecular Genetics and Molecular biology Department, Genetic engineering and Biotechnology Research Institute, Sadat City University, Egypt.

Email

-

City

Genetic engineering and Biotechnology Research Institute, Sadat City University, Egypt.

Orcid

-

First Name

Wael

Last Name

Roshdy

MiddleName

-

Affiliation

Central Public Health Laboratories, Ministry of Health and Population, Cairo, Egypt.

Email

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City

Ministry of Health and Population, Cairo, Egypt.

Orcid

-

First Name

Wael

Last Name

Abdel-Mageed

MiddleName

-

Affiliation

Genetics Department, Faculty of Agriculture, Beni-Suif University, Egypt

Email

-

City

Faculty of Agriculture, Beni-Suif University, Egypt

Orcid

-

Volume

27

Article Issue

1

Related Issue

28818

Issue Date

2022-01-01

Receive Date

2021-10-15

Publish Date

2022-01-01

Page Start

15

Page End

30

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 27_Issue 1_Pages 15-30.pdf

PDF . 781.8kB

Link

https://jpd.journals.ekb.eg/article_205853.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=205853

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2

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

NATURAL PRODUCTS EFFECT OF TREATMENTS ON GENE TARGETING METABOLISM ON BREAST CANCER

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Article

Created At

22 Jan 2023