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SDS-PAGE ELECTROPHORESIS AND SOLUBILITY ‎CHARACTERISTICS OF CASEIN – Chlorella Vulgaris PROTEIN ‎ISOLATE CO-PRECIPITATE MIXTURES

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Last updated: 04 Jan 2025

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Abstract

Protein isolate was obtained from microalgae Chlorella vulgaris, the protein percent in the isolated protein was 56%.  A solution (3.5% protein) of Chlorella vulgaris protein isolate (CPI) was blended with fresh skim milk (pH 6.6) at volume ratios of 5:95 , 10:90 and 15:85 (vol:vol) Chlorella protein solution to fresh skim milk and the protein mixtures were co-precipitated at pH4 using 2N-Hcl. The obtained three Casein – Chlorella protein co-precipitate mixtures were separated electrophoretically using SDS – PAGE. The molecular weights of most separated mixtures had intermediate values between molecular weight of cow′s milk casein and chlorella protein.
Solubilities of the obtained three mixtures were higher than that of the cow′s milk casein at pH6, ranged from 80 to 87.1% comparing with cow′s milk casein (60%).
Conclusively, these results suggested that, casein – chlorella protein mixtures may find their uses in acidic foods.
         

DOI

10.21608/jpd.2022.251625

Keywords

SDS-PAGE Electrophoresis, solubility characteristics, casein, Chlorella vulgaris, protein isolate, co-precipitate mixtures

Authors

First Name

Omaima

Last Name

Alattar

MiddleName

-

Affiliation

Department of Food and Dairy Technology. , Faculty of Technology and ‎Development, Zagazig University, Zagazig, Egypt.‎

Email

omaimaalattar4@gmail.com

City

Faculty of Technology and ‎Development, Zagazig University, Zagazig, Egypt.‎

Orcid

-

First Name

Hazem

Last Name

Fayed

MiddleName

-

Affiliation

Department of Food and Dairy Technology. , Faculty of Technology and ‎Development, Zagazig University, Zagazig, Egypt.‎

Email

-

City

Faculty of Technology and ‎Development, Zagazig University, Zagazig, Egypt.‎

Orcid

-

First Name

Abd ell naaby

Last Name

Farag‎

MiddleName

-

Affiliation

Department of Food and Dairy Technology. , Faculty of Technology and ‎Development, Zagazig University, Zagazig, Egypt.‎

Email

-

City

Faculty of Technology and ‎Development, Zagazig University, Zagazig, Egypt.‎

Orcid

-

Volume

27

Article Issue

2

Related Issue

28817

Issue Date

2022-04-01

Receive Date

2022-02-15

Publish Date

2022-04-01

Page Start

263

Page End

279

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 27_Issue 2_Pages 263-279.pdf

PDF . 869.0kB

Link

https://jpd.journals.ekb.eg/article_251625.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=251625

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5

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Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

SDS-PAGE ELECTROPHORESIS AND SOLUBILITY ‎CHARACTERISTICS OF CASEIN – Chlorella Vulgaris PROTEIN ‎ISOLATE CO-PRECIPITATE MIXTURES

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Article

Created At

22 Jan 2023