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181335

ACTIVITY STUDY OF FRESH AND FROZEN-THAWED SPERMATOZOA ON INTRACYTOPLASMIC SPERM INJECTION OUTCOME

Article

Last updated: 22 Jan 2023

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Abstract

To assess fertilization, implantation, and pregnancy rates in patients undergoing intracytoplasmic sperm inject (ICSI) using fresh and cryopreserved sperm from ejaculated semen samples.Retrospective study, from a total of 60 patients, 44 ICSI cycles were included in this study.Fertilization, implantation, and pregnancy rates were evaluated; 20 cycles of ICSI (cryopreserved-sperm group) and 20 cycles of ICSI (fresh-sperm group). Also, divided the outcomes according to the semen characteristics, normozoospermia, oligozoospermia, asthenozoospermia, and oligoasthenozoospermia.Overall, normal-fertilization rates were higher using fresh sperm compared with cryopreserved sperm.
 Cycles performed in patients with normozoospermia or oligozoospermia had similar fertilization, implantation, and pregnancy rates using fresh or cryopreserved sperm. When asthenozoospermic and oligoasthenozoospermic semen samples were used, the normal-fertilization rate was higher with fresh sperm compared with cryopreserved sperm. However, implantation and pregnancy rates were similar in fresh and cryopreserved sperm samples from patients with asthenozoospermia or oligoasthenozoospermia.
In conclusion, semen with abnormalities in the motility may be more susceptible to sperm cryopreservation damage, resulting in lower fertilization rates. However, once the oocyte is fertilized, implantation and pregnancy rates are similar to those in patients with oligozoospermia and normozoospermia.
       
 
 
 

DOI

10.21608/jpd.2021.181335

Keywords

Semen, cryopreservation, ICSI, Spermatozoa, fertilization

Authors

First Name

Mohamed

Last Name

Gad

MiddleName

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Affiliation

Obs.& Gyn Department, Faculty of Medicine, Menoufia University, Egypt

Email

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City

Faculty of Medicine, Menoufia University

Orcid

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First Name

Bahgat

Last Name

El Fiky

MiddleName

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Affiliation

Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt

Email

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City

Genetic Engineering and Biotechnology Research Institute

Orcid

-

First Name

Hanan

Last Name

El-Saiedy

MiddleName

-

Affiliation

Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Egypt

Email

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City

Genetic Engineering and Biotechnology Research Institute

Orcid

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First Name

Ebrahim

Last Name

Sabra

MiddleName

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Affiliation

Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Email

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City

Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt.

Orcid

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Volume

26

Article Issue

2

Related Issue

26098

Issue Date

2021-04-01

Receive Date

2021-02-15

Publish Date

2021-04-01

Page Start

311

Page End

323

Print ISSN

1110-2543

Online ISSN

2682-3322

Link

https://jpd.journals.ekb.eg/article_181335.html

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https://jpd.journals.ekb.eg/service?article_code=181335

Order

7

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023