ManageChatDiscover
203273

DEVELOPMENT OF RESISTANCE TO CHLORPYRIFOS-METHYL IN APHIS CRACCIVORA KOCH AND ITS IMPACT ON CERTAIN BIOLOGICAL ACTIVITIES

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

The present work is a trial to study the development and build up of resistance to chlorpyrifos-methyl in a field strain of Aphis craccivora  and to evaluate the cross resistance patterns to several conventional and novel insecticides. Additionally, to evaluate the effect of chlorpyrifos-methyl resistance on fitness cost of Aphis craccivora. With the completion of the work, the development of resistance to chlorpyrifos-methyl toxicity in Aphis craccivora as a result of insecticide selection pressure. The resistance level was amplified over time by continuing exposure to chlorpyrifos-methyl.The resistance ratio (RR) for the selected strain increased from 22.87-fold for the 1st generation to 45.11-fold for the 8th generation and 77.76-fold for the 15th generation. On other word, the oviposition period of the field strain (11.02 days) was longer as compared to resistant strain (6.54) which was shorter than susceptible strain (10.15).The longevity of the chlorpyrifos-methyl resistant strain was (20.68day) and was longer than susceptible aphids (15.24) but field strain was shorter (16.55day). Thefecundity was significantly low in resistant strain of Aphis craccivora. The intrinsic rate of increase (rm) ,the finite rate of increase (λ) and the net reproductive rate (R0) of the resistant strain of Aphis craccivora were significantly decreased comparing by susceptible strain. While, the mean generation time (T) in resistant strain was markedly increased as compared to the susceptible strain of Aphis craccivora. Results indicated that the resistant strain showed high resistance to malathion (27.75-fold) after the 8thgeneration, development of resistance increased to 39.94-fold after the15th generation; and the resistance to imidacloprid was 11.76-fold after the 8thgeneration then developed to 15.28-fold after the 15th generation. The pirimicarb , zeta-cypermethrin and other insecticides with novel mode of action showed no cross resistance with resistance ratio below 5-fold. So, they can be used in chlorpyrifos-methyl resistance management programs through rotation to reduce the possibility of resistance development in the field. Acetyl cholinesterase (AChE), carboxylesterase activity, Mixed function oxidase (MFO) and glutathione-s-transferase (GST) activity were also measured. Acetyl cholineesterase activity demonstrate a significant role for this enzyme in chlorpyrifos-methyl resistance but other secondary mechanism may be involved.            

DOI

10.21608/jpd.2021.203273

Keywords

Aphis craccivora Koch, Chlorpyrifos-methyl, Cross-resistance, Fitness cost, Enzymes

Authors

First Name

Rehab

Last Name

Abd-Allah

MiddleName

-

Affiliation

Plant Protection Research Institute, A.R.C., Dokki, Giza – Egypt.

Email

-

City

A.R.C., Dokki, Giza – Egypt.

Orcid

-

First Name

Abd El Hamid

Last Name

Mohanna

MiddleName

-

Affiliation

Department of Plant Production, Faculty of Technology & Development, Zagazig University, Egypt.

Email

-

City

Faculty of Technology & Development, Zagazig University, Egypt.

Orcid

-

First Name

Hamza

Last Name

El-Sharkawy

MiddleName

-

Affiliation

Department of Plant Production, Faculty of Technology &Development, Zagazig University, Egypt

Email

hamzash@hotmail.com

City

Faculty of Technology &Development, Zagazig

Orcid

-

First Name

Mostafa

Last Name

Hashem

MiddleName

-

Affiliation

Plant Protection Research Institute, A.R.C., Dokki, Giza – Egypt.

Email

-

City

A.R.C., Dokki, Giza – Egypt.

Orcid

-

Volume

26

Article Issue

4

Related Issue

20763

Issue Date

2021-10-01

Receive Date

2021-07-14

Publish Date

2021-10-01

Page Start

689

Page End

702

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 26_Issue 4_Pages 689-702.pdf

PDF . 774.5kB

Link

https://jpd.journals.ekb.eg/article_203273.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=203273

Order

8

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

DEVELOPMENT OF RESISTANCE TO CHLORPYRIFOS-METHYL IN APHIS CRACCIVORA KOCH AND ITS IMPACT ON CERTAIN BIOLOGICAL ACTIVITIES

Details

Type

Article

Created At

22 Jan 2023