An in vitro propagation was carried out at Plant Tissue Culture
Laboratory, Agricultural Development System Project (ADSP), Ministry of
Agriculture, during the successive seasons of 2004/2005 and 2005/2006, to
find out the favorable methodology for in vitro propagation of
Chrysanthemum morifolium, Ramat. The results could be summarized in the
following:
The largest shoot length and number of leaves /shoot tip or node
explants occurred due MS medium supplemented with 0.1 mg /L TDZ, whereas
shoot formation (%) was the best when shoot tips or nodes were subculture in
WPM medium supplied with 0.1mg /L TDZ, but callus formation favoured the
1.0 mg/l TDZ on WPM. Application GA3 at 1.0 mg /L to shoot tips, subculture
on MS medium supplemented with 1.0 mg BA /L., resulted the best shoot
length, number of leaves as well as shoot and callus formation. Applying 0.5
mg /L TDZ combined with 2.0 mg /L IAA to shoot tip explants gave the more
number of leaves and shoot formation. While 2.0 mg /L IAA alone on shoot
tips resulted the longest shoots. The heaviest callus formation occurred on
node explants treated with 0.5 mg TDZ/l.
Shoot tips subculture on MS medium supplemented with 1.0 mg/l BA
under light intensity of 1000 lux produced the larger number of leaves and
shoot length.
Callus formation was the heaviest on node explants under 1000 lux light
intensity. The largest number of leaves and shoot length occurred on shoot
tips subculture on MS medium supplied with 1.0 mg /L BA under 240C.
But,callus formation was the best on node explants under 17 0C. Adding
sucrose to MS medium supplemented with 1.0 mg /L BA was the best
compared to fructose and glucose as sources of carbon in producing shoot
length, number of leaves and callus formation. The highest values of
characters occurred due to 20 g /L sucrose combined with shoot tip explants.
Shoot length and number of leaves/ shoot tip or node explants were the largest
when treated with 1.0 mg /L BA, combined with 15 g. /L sucrose. Whereas
number of shoots on shoot tips, or node explants was the highest at 1.0 mg /L
BA with 10 g /L sucrose. The 1.0 mg /L IAA combined with 15 g /L sucrose led
to the heaviest callus formation. While root length was the longest by 0.1mg /L
BA combined with sucrose at 10 g /L. MS medium at 1/2 strength resulted the
largest number of roots, root length and root formation, While full strength of
MS failed to induce roots .Reducing MS strength caused concomitant decrease
in the growth; characters shoot length and number of leaves. MS salts medium
at full strength gave the largest growth characters.