This investigation was carried out in 2004 and 2005 at Plant Tissue
Culture Laboratory, Agricultural Development System Project (ADSP),
Ministry of Agriculture, to detect the effect of some media types, different levels
of some cytokinins and growth substances on some growth and rooting
parameters of Anthurium scherzeranum, Schott in vitro as well as the influence
of NPK fertilization on plantlets transferred from in vitro to in vivo greenhouse
conditions. The obtained results would be briefed in the following : Number of
shoots and leaves were significantly raised by N & N medium, supplemented
with 5.0mg/l for TDZ or BA individually. But, shoot length was significantly
increased by MS medium free from TDZ or BA supplementation, whereas, fresh
weight / shoot tip showed varied responses towards the two cytokinins. The MS
medium supplied with TDZ at 2.0 mg/l or 5.0 mg BA/l supplemented to the N &
N medium gave the heaviest fresh weight. Shoot length and fresh weight /shoot
tip were significantly increased by AgNO3 at 4-5mg/l or 2.0 mg GA3 /L
supplemented to MS medium, individually or in combination. Number of shoots
and leaves were significantly improved by 3.0 mg /L AgNO3, but they were not
affected by GA3 levels, individually; whereas 3.0 mg /L AgNO3 combined with
1.0mg GA3 /L significantly resulted the highest record of these two characters.
The half strength of MS, supplemented with 0.5 mg IBA /L combination,
significantly gave the best growth parameters (shoot length, number of leaves
and fresh weight). While root number and length were significantly increased
by 1.0 mg IBA/l added to 1/8 and 1/4 MS strengths, respectively. Plants
transferred from in vitro to in vivo under greenhouse conditions showed a
significant increase in number and length of shoots when fertilized with
N:P:K:Mg (19:19:19:32) at rate of 4.0 g /pot, while number of leaves and
flowers were significantly improved by 3.0 or 4.0 g /pot N: P: K (19:19:19),
consecutively, applied in two equal doses.