The in vitro growth and development of Dutch Iris cv. Blue Magic plants is determined by a number of complex factors including nutrients, sucrose and plant growth regulators . The effect of cytokinins ( BA ) and auxin ( NAA ) as well as type of medium (MS, B5 and WPM )on in vitro growth were studied multiplication stage while plant growth retardants ( CCC,PP333 and ABA) and sucrose were studied too during bulb formation stage. The effect of BA and NAA combinations on the production of Dutch Iris cv. Blue Magic bulbs from shoots in vitro was investigated . Combinations between BA at 1.0or 2.0mg /l and NAA at 0.2 mg/l were the best medium for shoot formation estimated as number of shoot, shoot length, number of leaves and fresh weight. Shoot formation of Iris was affected by MS, B5 and WPM media. Plantlets cultured on MS medium recorded the best shoot formation for shoot formation followed by B5 and WPM media. At bulblet formation , the highest number of bulbs and bulbs percentage were only obtained with the medium contained CCC at 25 µM, while the highest number of shoots and leaves were recorded when the medium contained 5,10,20,and 25 µM ABA compared to other treatments. The effect of sucrose concentrations (30, 60 and 90 g/l) on blublet formation were studied. MS nutrient media containing 25 µM PP333 and 90g/l sucrose increased significantly the average number of bulbs/shoot and diameter of bulbs. Plantlets derived from 90g/l sucrose containing 25 µM PP333 showed the highest percentage of survival (90%) at acclimatization stage. The developed shoots were transferred to a rooting medium containing two types of auxins ( IBA or NAA each at 0.0, 1.0, 2.0 and 3.0 mg/l ). The treatment of MS medium containing 2 mg/L NAA produced the largest number of roots as well as root length. Plantlets grown on MS rooting medium supplemented with 2.0mg/l NAA showed the highest percentage of survival (85%)during acclimatization stage. In this work, the soil mixture used in acclimatization consisted of peatmoss : perlite (2:1v/v).