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44896

BIOTECHNOLOGICAL STUDIES ON Jatropha curcas L., AN IMPORTANT BIOFUEL PLANT

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Last updated: 04 Jan 2025

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Abstract

Techniques for the induction of callus from petiole and leaf explants of Jatropha curcas (L) have been developed. Callus induction from petiole and leaf explants was evaluated on a range of N6 benzyl adenine (BA) and α-naphthalene acetic acid (NAA) concentrations.
 Both explants were capable to form100% callus, in different levels. Callus formation and growth were significantly affected by source of explants and growth regulators. The highest amount of callus was induced from leaf segments on MS solid medium supplemented with 0.5 mg/l of BA plus 0.5 mg/l of NAA. Callus-derived shoots were strongly affected by the residual effect of growth regulators levels. Callus derived from third leaf showed the highest shoot numbers and percentage of shoot formation (90%) when cultured on MS solid medium contained 0.1 mg/l of BA. The produced callus on 0.1 mg/l BA plus 1.0 mg/l indole butyric acid (IBA) was dedifferentiated when transferred into MS medium contained 0.1 mg/l of BA. Different extracts of Jatropha curcas (L) derived from in vivo and in vitro were investigated.
      

DOI

10.21608/jpd.2008.44896

Keywords

Jatropha curcas, Ephorbaceae, growth regulators, explant type, in Vitro, Callus, anti-tumor activities

Authors

First Name

Metwally

Last Name

Bekhit

MiddleName

-

Affiliation

Plant Biotechnology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Branch of Sadat, Sadat City, Egypt.

Email

-

City

GEBRI, Minufiya University

Orcid

-

First Name

Manal

Last Name

Solieman

MiddleName

-

Affiliation

Plant Biotechnology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Branch of Sadat, Sadat City, Egypt.

Email

-

City

GEBRI, Minufiya University

Orcid

-

First Name

Mostafa

Last Name

Abd Elkawi

MiddleName

-

Affiliation

Faculty of pharmacy, Cairo University, Egypt

Email

-

City

Cairo University, Egypt

Orcid

-

First Name

Ibrahim

Last Name

Abd El Maksoud

MiddleName

-

Affiliation

Plant Biotechnology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Branch of Sadat, Sadat City, Egypt.

Email

-

City

sadat city

Orcid

-

First Name

Khalil

Last Name

Elhalafawi

MiddleName

-

Affiliation

Molecular Biology Dept., Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Branch of Sadat, Sadat City, Egypt.

Email

-

City

GEBRI, Minufiya University,

Orcid

-

Volume

13

Article Issue

2

Related Issue

6947

Issue Date

2008-07-01

Receive Date

2008-06-16

Publish Date

2008-07-01

Page Start

443

Page End

455

Print ISSN

1110-2543

Online ISSN

2682-3322

Link

https://jpd.journals.ekb.eg/article_44896.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=44896

Order

14

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

BIOTECHNOLOGICAL STUDIES ON Jatropha curcas L., AN IMPORTANT BIOFUEL PLANT

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Article

Created At

22 Jan 2023