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44679

CLONE PRODUCTION FROM PELARGONIUM GRAVEOLENS L,HERIT. TOLERANT TO WILT THROUGH TISSUE CULTURE

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Last updated: 22 Jan 2023

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Abstract

      This work was carried out in the plant tissue culture laboratory of the Vegetable and Medicinal Plant Research Department, Horticulture Research Institute, Ministry of Agriculture, during the period of 2004 to 2008 to produce geranium (Pelargonium graveolens L, Herit.) clones that tolerate to fusarium wilt through tissue culture. The study included three items:
1- Callus induction, callus differentiation and acclimatization.
2- Fungal infection of fusarium in the pots of the tested clones.
3- Evaluation of clones compared with mother plants.
- Wilt percentage after the plants were infected with fusarium fungal
- Morphological characteristics for clones and mother plants and essential oil percentage & constituents.
- DNA and protein isolationfor clones and mother plants
The obtained results were as follow:-
- Obtaining of two clones from Pelargonium graveolens L, Herit., tolerate to fusarium wilt
- There were true significant different between two clones and mother plant in leaves area characteristics and oil percentage
- There were variation in DNA isolation between two clones and mother plant
As such we recommended propagating this clones and increasing its cultivated area to come over geranium wilt disease.
 
 
 

DOI

10.21608/jpd.2009.44679

Keywords

DNA, callus induction, Pelargonium graveolens, Evaluation, mother plants

Authors

First Name

Ayman

Last Name

Hamouda

MiddleName

-

Affiliation

MAPS Department, Horticulture Research Institute, Agriculture Research Center, Giza, Egypt.

Email

-

City

ARC, Giza

Orcid

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First Name

Ashraf

Last Name

Abd-Elrazek

MiddleName

-

Affiliation

Genetic Department, Faculty of Agriculture, Ein-Shams Unerivsity, Egypt.

Email

-

City

Ein-Shams Unerivsity

Orcid

-

First Name

Mohamed

Last Name

Baiuomy

MiddleName

-

Affiliation

Plant Pathology Research Institute, Agric. Res. Center, Giza, Egypt.

Email

-

City

ARC,Giza

Orcid

-

Volume

14

Article Issue

2

Related Issue

6932

Issue Date

2009-04-01

Receive Date

2019-08-13

Publish Date

2009-04-01

Page Start

339

Page End

356

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 14_Issue 2_Pages 339-356.pdf

PDF . 463.4kB

Link

https://jpd.journals.ekb.eg/article_44679.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=44679

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5

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023