Beta
ManageSearchChatDiscover
42464

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION INDUCED FROM MATURE SEEDS, COTYLEDONS AND SHOOT TIPS OF CUCUMBER.

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Commercial cucumber cultivars were explored for embryogenesis and plant regeneration induced in somatic tissues on plant growth regulators.  An efficient in vitro regeneration protocol for Cucumis sativus L via somatic embryogenesis has been developed. Embryogenic callus  cultures  were  established  from  mature seeds, cotyledons and shoot tips  on Murashige and Skoog (MS) medium  containing  87.64  µM sucrose, 0.8% agar, 2,4-dichlorophenoxyacetic acid 2,4-D and kinetin.  Maximum callus induction (94%, 92%) was observed in cotyledons and mature seeds on MS medium supplemented with 1mg/L 2,4-D, respectively. Somatic embryos were observed on mature seed explants after 17 weeks on MS medium supplemented with 5mg/L2,4-D (33%) and 0.5mg/L α-naphthaleneacetic acid(NAA) (27%). while somatic embryos were observed on cotyledon explants after 18 weeks on MS medium supplemented with 2mg/L 2,4-D (10%) and 0.5mg/L NAA (25%). The highest percentage of somatic embryogenesis (40%) was obtained with 2 mg/L 2,4-D and 0.5 mg/L NAA (30%) on shoot tip explants after 13 weeks. Regeneration of adventitious buds from callus of cotyledon and mature seeds were achieved after 8 weeks on MS medium supplemented with 0.5mg/L and 1mg/L 6-benzyladenine(BA), respectively. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil. Generally, auxin was found critical for induction of callus and formation of somatic embryos, while the cytokinin was essential for callus differentiation and plant regeneration.  

DOI

10.21608/jpd.2012.42464

Keywords

Cucumber, Cytokinin, somatic embryogenesis, cotyledon, callus, Cucumis sativus L., shoot tip

Authors

First Name

Yehia

Last Name

Khidr

MiddleName

-

Affiliation

Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University

Email

yehia.khidr@yahoo.com

City

Sadat city

Orcid

-

First Name

El-Sayed

Last Name

El-Absawy

MiddleName

-

Affiliation

Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University

Email

-

City

Sadat city

Orcid

-

First Name

Amal

Last Name

Mahmoud

MiddleName

-

Affiliation

Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Hasan

MiddleName

-

Affiliation

Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University

Email

-

City

Sadat city

Orcid

-

First Name

Alaa

Last Name

Hemeida

MiddleName

-

Affiliation

Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute, Sadat City, Menofia University

Email

-

City

Sadat city

Orcid

-

Volume

17

Article Issue

2

Related Issue

6659

Issue Date

2012-04-01

Receive Date

2012-03-07

Publish Date

2012-04-01

Page Start

193

Page End

209

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 17_Issue 2_Pages 193-209.pdf

PDF . 447.1kB

Link

https://jpd.journals.ekb.eg/article_42464.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=42464

Order

3

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023