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42457

VIABILITY OF BISECTED RABBIT EMBRYOS AFTER CRYOPRESERVATION USING VITRIFICATION

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Last updated: 22 Jan 2023

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Abstract

To study the effects of developmental capacity after in vitro culture of intact and demi-embryos of rabbit in fresh case or after cryopreservation using vitrification technique, total 89 embryos at morula and blastocyst stages recovered from 16 does. Compaction rabbit embryos maintained intact (control) were compared with bisected embryos using a simplified splitting protocol at fresh or vitrified thawed embryos. The survivability of intact embryos and demi-embryos were evaluated after 24 hours of in vitro culture by development ability and morphological assessment. The percentage of development after 24 h of in vitro culture was higher (P<0.05) in fresh intact embryos than in fresh bisected embryos, either at morula (80 vs. 56%) or blastocyst (84.6 vs. 63.6%) stages. In case of intact or bisected embryos, percentage of development after 24 h of in vitro culture was insignificantly higher in embryos at blastocyst than at morula stages. The percentage of development after 24 h of in vitro culture was higher (P<0.05) for vitrified intact embryos than for vitrified bisected embryos, either at morula (61.5 vs. 45.0%) or blastocyst (75.0 vs. 42.1%) stages. In case of intact embryos, percentage of development after 24 h of in vitro culture was higher (P<0.05) for embryos at blastocyst than at morula stages. However, the percentage of development after 24 h of in vitro culture was nearly similar in vitrified bisected embryos, either at morula or at blastocyst stages.
In conclusion, bisection and vitrification can be used for rabbit embryos, but embryonic stage and cryopreservation are limiting factors in the success of these procedures.
 
 

DOI

10.21608/jpd.2012.42457

Keywords

Rabbit, embryo, bisection, developmental capacity, In vitro culture

Authors

First Name

Ahmed

Last Name

Hussein

MiddleName

-

Affiliation

Animal Production Research Institute, Agriculture Research Center, Giza, Egypt.

Email

-

City

Giza

Orcid

-

First Name

Mohamed

Last Name

El-Siefy

MiddleName

-

Affiliation

Animal Production Research Institute, Agriculture Research Center, Giza, Egypt.

Email

-

City

Giza

Orcid

-

First Name

Mohamed

Last Name

Omara

MiddleName

-

Affiliation

Animal Production Research Institute, Agriculture Research Center, Giza, Egypt.

Email

-

City

Giza

Orcid

-

First Name

Mohamed

Last Name

El-Sherbieny

MiddleName

-

Affiliation

Animal Production Research Institute, Agriculture Research Center, Giza, Egypt.

Email

-

City

giza

Orcid

-

Volume

17

Article Issue

1

Related Issue

6657

Issue Date

2012-01-01

Receive Date

2011-10-09

Publish Date

2012-01-01

Page Start

65

Page End

77

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 17_Issue 1_Pages 65-77.pdf

PDF . 266.3kB

Link

https://jpd.journals.ekb.eg/article_42457.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=42457

Order

4

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023