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NEW ALTERNATIVE METHOD OF DRUMSTICK TREE (Moringa oleifera Lam.) MICROPROPAGATION.

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Last updated: 04 Jan 2025

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Abstract

Tissue culture protocol reported in this study is an alternative method suggested to produce plantlets of drumstick tree (Moringa oleifera Lam.) with uniform genotypes for breeding, in vitro and in vivo production. The highest rate of micropropagtion will often depend not only on the selection of the most suitable explants, but also on sterilization agent and the best basal medium for that tissue. Sterilizing shoot tip explants of drumstick, using 15 % of clorex for 15 minutes showed the highest survival percentage and lowest contamination as well as sufficient browning percentage.  For multiple shoot induction, the shoot tip explants of drumstick were cultured on Murashige and Skoog (MS) agar medium supplemented with different concentrations  of  2-isopentinyl adenine (2iP) at (0.0,0.5,1.0 and 2.0 mg/L) alone or in combination with indol butyric acid (IBA) at ( 0.0,0.1,0.5and 1.0 mg/ L). The highest shoot multiplication rate was found in the 3rd subculture.  Moreover, the highest  shoot  number/explant (8.8)  was  obtained  with  the medium contained  0.5mg/l  2iP with 0.1mg/ L   IBA and maintained at 25±2C  compared  to all other treatments. The control treatment alone significantly showed the highest shoot length compared to all other treatments Low concentrations (0.5mg/L ) with (0.1ml/L IBA) were more effective in increasing the number of leaves. Plantlets grown on the control medium produced the maximum number of healthy roots. The rooted plantlets were hardened and successfully established in the soil.    

DOI

10.21608/jpd.2013.42580

Keywords

Moringa oleifera Lam, drumstick tree, shoot tip, sterilization, shoot multiplication

Authors

First Name

khaled

Last Name

Mazrou

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI). Sadat City University, Sadat City, Egypt.

Email

mostaw100@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Atfi

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI). Sadat City University, Sadat City, Egypt.

Email

-

City

Sadat city

Orcid

-

Volume

18

Article Issue

3

Related Issue

6656

Issue Date

2013-08-01

Receive Date

2013-05-02

Publish Date

2013-08-01

Page Start

339

Page End

348

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 18_Issue 3_Pages 339-348.pdf

PDF . 324.8kB

Link

https://jpd.journals.ekb.eg/article_42580.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=42580

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Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

NEW ALTERNATIVE METHOD OF DRUMSTICK TREE (Moringa oleifera Lam.) MICROPROPAGATION.

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Article

Created At

22 Jan 2023