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42258

EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION FROM SEEDLING EXPLANTS OF Ammi visnaga L. AND PHENOLIC COMPOUNDS CONTENT

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Last updated: 04 Jan 2025

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Abstract

Ammi visnaga (L.) Lam. Belong to family Umbelliferae. During the present study an efficient in vitro protocol has been standardized viz, callus production from seedling explants. Best callus production from seedling explants was obtained on MS medium supplemented with 0.5mg/l NAA +0.05/1BA. Callus was also obtained when MS medium was fortified with 1mg/1NAA+0.5mg/1BA, 1mg/1 (2, 4D+0.5 mg/1BA and 3mg/l PCIB. But the percent culture response on these concentrations was lesser. The lowest amount of callus was found to be on MS medium containing 1mg/l PCIB 9(1) callus amount.
Callus of explants were grown on half MS media gave the significant highest value (3641) of phenolic content, followed by callus of free-growth regulators MS medium (3509.5). While the lowest value (7) was observed with callus obtained from seedling cultured on MS medium contained 1 mg/1 2, 4-D+ 0.5 mg/1K.
Conclusively, the Ammi visnaga callus formation can be obtained from different concentrations of growth regulators. The growth regulators also had a significant impact on the amount of callus produced.The efficiency of callus formation depended on the hormone concentrations and the proportion between NAA and BA. The best results were achieved on the medium containing 1mg/lNAA and 0.05mg/lBA. The callus of Half MS plant gave the significant highest value of phenolic content, followed by callus of Free-growth regulators MS medium.
 

DOI

10.21608/jpd.2016.42258

Keywords

Ammi visnaga (L.), NAA, PCIB, callus formation, 2, 4-D and phnolic compounds

Authors

First Name

Sayed

Last Name

Hussein

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt

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City

sadat city

Orcid

-

First Name

Heba

Last Name

Shahin

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt

Email

-

City

sadat city

Orcid

-

First Name

Yasseen

Last Name

Yasseen

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt

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-

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Volume

21

Article Issue

1

Related Issue

6633

Issue Date

2016-01-01

Receive Date

2015-09-20

Publish Date

2016-01-01

Page Start

55

Page End

66

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 21_Issue 1_Pages 55-66.pdf

PDF . 731.8kB

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https://jpd.journals.ekb.eg/article_42258.html

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https://jpd.journals.ekb.eg/service?article_code=42258

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Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

EFFECT OF PLANT GROWTH REGULATORS ON CALLUS INDUCTION FROM SEEDLING EXPLANTS OF Ammi visnaga L. AND PHENOLIC COMPOUNDS CONTENT

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Article

Created At

22 Jan 2023