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42806

DEVELOPMENT OF HAPLOID PLANTS THROUGH OVARY CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L ).

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Last updated: 22 Jan 2023

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Abstract

This research was done at department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute( GEBRI ), Sadat City university, Menufyia, Egypt from 2009 to 2014 years to study the effect of four factors affected  callus induction of ovary cultur i.e.(cold pretreatment, chemical pretreatments, growth regulators and spikes sources. and establishment a technique to produce haploid plants. Three wheat varieties were used, Sids 1, Gemmeiza 9 and Giza 168. The results indicated that, chemical pretreatments by sterilization substances (Ethanol alcohol 70% or Mercurec chloride 1g/L or Thiourea 2g/L) were very important and essential to induce calli from ovaries. The combination between (1 and 2 mg /L 2,4-D ) and (0 , 0.1 and 0.2 mg/L kinetien) gave the highest response of callus induction and plant regeneration percentages. The estimates were increased and developed with increasing  the cultured area which the spiks were collected from it. The estimates were varied with varieties.  

DOI

10.21608/jpd.2015.42806

Keywords

Wheat, Ovary culture, Cold & chemical pretreatments, growth regulators, Source of spikes, varieties

Authors

First Name

Haron

Last Name

Abo Shama

MiddleName

-

Affiliation

Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt

Email

-

City

Sadat City

Orcid

-

First Name

Hala

Last Name

Ghareeb

MiddleName

-

Affiliation

Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt.

Email

-

City

Sadat city

Orcid

-

First Name

yasin

Last Name

yasin

MiddleName

-

Affiliation

Plant Biotech. Department Genetic Engineering and Biotech. Research Institute ( GEBRI ), Sadat City. Univ. Menufyia, Egypt.

Email

-

City

Sadat city

Orcid

-

Volume

20

Article Issue

3

Related Issue

6563

Issue Date

2015-08-01

Receive Date

2015-06-22

Publish Date

2015-08-01

Page Start

297

Page End

306

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 20_Issue 3_Pages 297-306.pdf

PDF . 664.9kB

Link

https://jpd.journals.ekb.eg/article_42806.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=42806

Order

4

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023