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42802

DEVELOPMENT OF HAPLOID PLANTS THROUGH ANTHER CULTURE TECHNIQUE IN WHEAT ( Triticum aestivum L. )

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Last updated: 22 Jan 2023

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Abstract

Anther culture was utilized to develop haploid plants in wheat .This study was carried out at Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute ( G E B R I ) from  2009  to  2014  years .  Three varieties of wheat were used , Sids1, Gemmeiza 9  and Giza 168. The studied factors were, cold and chemical pretreatments, growth regulators concentrations of 2,4-D and kinetin and effect of  source of spikes . The results indicated that the cold pretreatment had no effect on anther response of three wheat varieties, the chemical pretreatments by sterilization  substances (ethanol alcohol 70 % or mercurec chloride 1g /L or thiourea 2 g /L ) were very important and essential to induce callus from anther . The three treatments of ( 1mg /L 2,4-D ) , ( 2 mg /L 2,4-D ) and ( 2 mg /L  2,4-D + 0.1 mg /L kinetin ) gave the highest response of callus induction and plant regeneration . Androgenesis improved with increasing the cultured area which the spikes were collected from it .  

DOI

10.21608/jpd.2015.42802

Keywords

Wheat, Anther culture, Cold & chemical pretreatments, growth regulators, Effect of source of spikes, varieties

Authors

First Name

Haron

Last Name

Abo Shama

MiddleName

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Affiliation

Plant Biotech Dept . Genet. Engin. and Biotech .Res. Inst ( GEBRI), Sadat City, University Menufiya, Egypt.

Email

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City

Sadat City,

Orcid

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First Name

Hala

Last Name

Ghareeb

MiddleName

-

Affiliation

Plant Biotech Dept . Genet. Engin. and Biotech .Res. Inst ( GEBRI), Sadat City, University Menufiya, Egypt.

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-

City

Sadat City

Orcid

-

Volume

20

Article Issue

3

Related Issue

6563

Issue Date

2015-08-01

Receive Date

2015-05-25

Publish Date

2015-08-01

Page Start

285

Page End

296

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 20_Issue 3_Pages 285-296.pdf

PDF . 688.8kB

Link

https://jpd.journals.ekb.eg/article_42802.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=42802

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3

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

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Article

Created At

22 Jan 2023