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42778

IMPROVED MICRO PROPAGATION OF Cichorium intybus L. FROM LEAF EXPLANT

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Last updated: 04 Jan 2025

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Abstract

Cichorium intybus is edible, medicinal and forage plant. The pharmaceutical raw materials were obtained from wild chicory (var. Maurane). Currently, farmers are increasingly assuming plantations of wild chicory, and breeders are attempting to produce cultivars for medicinal purposes. In the modern breeding of chicory important feature is the ability to propagate in vitro culture. The aim of our study was to assess capacity of natural population of wild chicory for plant regeneration from leaf explants. In the first was examined the effect of various concentrations of BA and TDZ on the regeneration of shoots from leaf explants (0.5 cm2). After that, 25 plants were propagated on the medium which was found as optimal. Then, their callus growth and shoots regeneration capacities were compared. The majority of the shoots were regenerated from callus but direct organogenesis was also observed (8%). Shoot regeneration was found to be the most efficient on MS medium containing 5MµBA and 3MµTDZ –97% of the explants produced shoots, while the average number of shoots was 15.5. The wild chicory shoots rooted easily.  

DOI

10.21608/jpd.2015.42778

Keywords

Cichorium intybus, Regeneration, TDZ, BA, callus formation and 2, 4-D

Authors

First Name

Heba

Last Name

Shahin

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, , P.O. Box 79/22857, Egypt.

Email

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City

Sadat City

Orcid

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First Name

Yasseen

Last Name

Yasseen

MiddleName

Mohamed

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt.

Email

-

City

Sadat City

Orcid

-

First Name

Mohamed

Last Name

Fahim

MiddleName

-

Affiliation

Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University, Sadat City, P.O. Box 79/22857, Egypt.

Email

-

City

Sadat City

Orcid

-

Volume

20

Article Issue

2

Related Issue

6562

Issue Date

2015-04-01

Receive Date

2015-02-10

Publish Date

2015-04-01

Page Start

139

Page End

148

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 20_Issue 2_Pages 139-148.pdf

PDF . 416.1kB

Link

https://jpd.journals.ekb.eg/article_42778.html

Detail API

https://jpd.journals.ekb.eg/service?article_code=42778

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2

Type

Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

IMPROVED MICRO PROPAGATION OF Cichorium intybus L. FROM LEAF EXPLANT

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Article

Created At

22 Jan 2023