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41328

EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.

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Last updated: 04 Jan 2025

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Abstract

This study was carried out to investigate the effect of addition of different levels of L-arginine (0, 3, 5, 7 and 10 mM) to improve the poor motile of Holstein bulls spermatozoa. Semen collected from five Holstein bulls once weekly and ejaculates with 45-50% mass motility was pooled. Semen was diluted with Tris- egg yolk extender at 37C° and divided into five parts,1st  part without supplement (control), other four parts 2nd , 3rd , 4th  and 5th were supplemented with 3, 5, 7 and 10 mM L-arginine respectively. Diluted semen was equilibrated at 4 C° for 4 h, then, filled in 0.25 ml French straws. Straws were kept on liquid nitrogen vapors for 10 min and then plunged and stored in liquid nitrogen at -196C°. Progressive sperm motility, live sperm, abnormal sperm and sperm acrosome integrity were determined post-dilution, equilibration and post-thawing processing.
The results were referred that extenders with concentrations of 5 and 7mM L-Arginine were improved all parameters studied than 3; 10 mM L-arginine extenders and control extender at different cryopreservation processes. The percentage of DNA damage were 20.0, 10.8, 4.40, 3.20 and 9.40 for control and 3, 5, 7 and 10 mM L-arginine extenders respectively. Significant differences in chromatin integrity were observed among  control and treatments (P
Conclusively, it could be concluded that adding L-arginine with  concentrations of 5 or 7mM to Tris-extender improved the freezability and fertility of poor quality of Holstein bull spermatozoa.
  
 

DOI

10.21608/jpd.2019.41328

Keywords

L- Arginine, semen extender, Poor quality Semen, DNA damage, Holstein bull

Authors

First Name

Emad

Last Name

Abd-Allah

MiddleName

-

Affiliation

Biotechnology Res. Dep. Animal Production Res. Institute, Agric. Res. Center, Egypt.

Email

-

City

Giza

Orcid

-

First Name

Entsar

Last Name

E. Z. Eliraqy

MiddleName

-

Affiliation

Biotechnology Res. Dep. Animal Production Res. Institute, Agric. Res. Center, Egypt.

Email

-

City

Kafr Elsheikh

Orcid

-

First Name

Mogda

Last Name

Salem

MiddleName

-

Affiliation

Animal Production Res. Institute, Agric. Res. Center, Egypt

Email

-

City

Giza

Orcid

-

Volume

24

Article Issue

1

Related Issue

6520

Issue Date

2019-01-01

Receive Date

2018-09-22

Publish Date

2019-01-01

Page Start

165

Page End

179

Print ISSN

1110-2543

Online ISSN

2682-3322

Article File

JPD_Volume 24_Issue 1_Pages 165-179.pdf

PDF . 494.1kB

Link

https://jpd.journals.ekb.eg/article_41328.html

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https://jpd.journals.ekb.eg/service?article_code=41328

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Original Article

Type Code

867

Publication Type

Journal

Publication Title

Journal of Productivity and Development

Publication Link

https://jpd.journals.ekb.eg/

MainTitle

EFFECT OF ADDITION L-ARGININE TO EXTENDER OF POOR MOTILE HOLSTEIN BULLS SPERMATOZOA.

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Article

Created At

22 Jan 2023