Background: COVID-19 vaccine hesitancy is a great challenge to control the current pandemic.Aim: Study aims to study COVID-19 vaccine hesitancy; establishing a base line to accelerate vaccine uptake and restore normal life in Beni-Suef City.  Subjects and method: Design: Cross-sectional design was used. Setting: The current study was performed in Beni-Suef city. Subjects: A convenient sample consists of 800 participants was chosen. Tools: The first was structured interview composed of 6 parts o assess, personal data, medical history, barriers, suggestions, willing to take the vaccine and knowledge about COVID-19 vaccine. The second was attitude scale used to assess the attitude regarding COVID-19 vaccine. Results: The data showed that friends were the main source of information for 38.7% of the studied sample while health team was the main source foronly 8.7% of the studied sample. Only 31% have satisfactory knowledge, 67% have negative attitude regarding the vaccines. 73.1% not willing to take the vaccine. Conclusion: The current study concluded that the total level of knowledge, attitude and willing to take the vaccine is low among the studied sample. Additionally the total level of knowledge, total level of attitude, medical history of chronic diseases age, education and residence were significantly associated with the willing of studied sample to take the vaccine.  Recommendations: Nursing staff and health team members should be involved in health education programs via mass media and via personal communications. Further studies aimed at improving population awareness and attitudes regarding COVID-19 vaccines.