Background: Mothers are the primary caregiver for children with stoma. Aim: Determine the effect of health care program for mothers having children with stoma. Subject and method: Aquasi-experimental design is used. The study was carried out for three months in the inpatient of the pediatric surgery department at El-Mansoura University Pediatric Hospital. Subject: A convenience sample of 52 mothers with stoma-affected children. Tools: Data was collected using three tools: (1) a structured interview questionnaire sheet that illustrated the personal characteristics of children with stomas and their mothers (II): Knowledge sheet of mothers about stoma, (III): Observational checklist form to examine mothers' practices about stoma care. Results: The mothers' knowledge and practices about stoma improved to 94.2%, 73.1 % respectively after program implementation with a high statistical significance difference p value < 0.001. Conclusion: The health-care program improved mothers' knowledge and practices of stoma care significantly. Recommendation: Implementation of periodic health care programs for mothers of children with stomas to improve their knowledge and practices on stoma care