Background: The gynecological examination is an essential part and the most performed procedure in gynecological care, that the maternity nurses play a vital role during it. Aim: was to assess the effect of gynecological examination educational guidelines on maternity nurses' knowledge, practices and explore the women's satisfaction. Methodology:  A quasi-experimental design was utilized for all nurse-midwife working at Al-Azhar university hospital, and El Zarka Central Hospital in Damietta Governorate. A total of 40 nurses were recruited in the study and attended the educational program. A total of 120 women were recruited to explore their Satisfaction regarding gynecological examination care. Three tools were utilized for data collection first, structured interviewing questionnaire, observational checklist, and women's' satisfaction assessment tool. The Results revealed that: the nurses mean age was 30.7 ± 3.10 . post-intervention most of maternity nurses  87.5%  had correct knowledge about gynecological examinations, 85% of them had satisfactory care about gynecological examinations. Almost all studied women  95% were satisfied with nursing care post-intervention Conclusion: The implemented educational guideline had a positive effect as it improves the nurses' knowledge and practice about gynecological examinations which inturn women were satisfied regarding care provided during gynecological examination. Recommendations: A training guideline regarding gynecological examination must be recommended for all nurses working at obstetric and gynecological units to improve the quality of care given to women. Also, further research to examine teaching vulvar self-examination and its implication for women at reproductive age.