Background: Workplace bullying is common problem that threatens nursing profession, nurse's health and ability to work safely. Aim: to assess workplace bullying among nurses in primary health care centers in Port Said governorate.Setting: Study was conducted in seven primary health care centers representing districts of Port Said governorate.Sample: composed of 61 staff nurses working at health clinics in the randomly selected primary health care centers. Tools of data collection: Data were obtained through Negative Acts Questionnaire-Revised (NAQ-R).Results: Majority of staff nurses (86.9%) had secondary nursing education and 82% were married. More than half of the studied nurses (54.1%) were victims of workplace bullying, two third of nurses (60.6%) had bullied by patients and patient relatives and more than one third (33.3%) bullied by supervisor and manager. Conclusion: It was concluded from this study, more than half of nurses were victims of workplace bullying. There was no statistical significant relation between workplace bullying and socio-demographic data.Recommendation: Counseling sessions about consequences of workplace bullying should be held on regular base in health care centers.