Background:Tactile stimulation has a great effect on decreasing neonatal stress during invasive procedures in neonatal intensive care units. Aim of the study: The study aimed to assess the effect of tactile stimulation on neonatal stress during invasive procedures at neonatal intensive care units. Design and setting: A quasi-experimental research design was used in this study, which was implemented in neonatal intensive care units Port Said, Port-Fouad and El-Nasr General Hospitals in Port Said. Sample: A sample of 130 neonates were used in the study. Tools: Data were collected by using three tools the first for neonatal characteristics, the second for assessment of neonatal blood sample (invasive procedure) and the third for stress physiological, behavioral & motor measurements. Results: There were high statistically significant differences in physiological, behavioral & motor measurements between two methods (with & without tactile stimulation) and the degree of neonatal stress control during invasive procedures in NICUs. Moreover, there was high statistically significant differences between two methods (with & without tactile stimulation) regarding total stress score and the degree of neonatal stress control during invasive procedures in NICUs, p value <0.001. Conclusion: The application of tactile stimulation has a positive effect on neonatal degree of stress among daily painful invasive procedures in NICUs. Recommendations: Continues implementing training program on tactile stimulation technique as a technical skill for nursing staff and implement further studies to investigate the effect of tactile stimulation on stress in different settings.