Background: Peer teaching is considered as an effective educational approach for nursing students. It prepares them for their future roles as educators, enhances their psychomotor skills, attitudes, and builds their self-esteem. Aim: The study aimed to evaluate the effect of peer teaching on nursing students' performance of clinical training in hemodialysis unit. Design: A quasi experimental research design was utilized to achieve the aim of this study. The current study was carried out at the two hemodialysis units of Mansoura university hospital. A convenient sample of 74 of second level nursing students who were enrolled in Medical surgical nursing course. They were assigned randomly into study group (peer learners) who were exposed to peer teaching method (under researchers' observations) and control group (instructor learners) who were exposed to instructor teaching. Six students (peer teachers) were selected, had previous experience of the hemodialysis clinical area with satisfied performance score prior to study. Tools: Three tools were utilized; Interview questionnaire sheet, Performance Observational Checklist, and Acceptance & satisfaction likert scales. Results: The results showed that there was no statistically significance difference between peer teaching group and control group regarding both of knowledge and performance concerning hemodialysis session termination. Conclusion: Peer teaching method can be used in clinical practice to overcome large numbers of students. Recommendations: Applying peer teaching method in clinical training. Also, it can be recommended to replicate the study in different clinical practice areas and large students' sample.