Background: The environment of work and culture of patient safety are crucial factors that had a major effect on missing nursing care. Aim: To explore the relationships between the work environment, patient safety culture, and missed nursing care among staff nurses. Method: Descriptive correlational design was used. The study included a convenience sample of 136 staff nurses who were working at all inpatient units of main Mansoura university hospital. Work environment scale, patient safety culture scale, and missed nursing care scale were utilized for data collection. Results: Staff nurses perceived average work environment, good patient safety culture, and low missing nursing care. The work environment and patient safety culture perceptions correlated negatively with missed nursing care. The work environment correlated positively with the culture of patient safety. Conclusion: Work environment and patient safety culture correlated negatively with missed nursing care among staff nurses. There was a positive relationship between work environment, and patient safety culture among staff nurses. Recommendations: Nurse Managers should maintain an effective work environment with adequate staffing, resources, and encourage teamwork to avoid missing nursing care. Nurse managers should continuously monitor and evaluate patient safety culture aspects among nurses to achieve a healthy and safe climate for patients. Clear communication and feedback system should be maintained about the error to avoid reoccurrence, and prevent its negative consequences for nurses, patients, and health care organizations