Background: care of wounds is a dynamic, complex process and requires specific knowledge of the nursing team, comprising professionals who will develop this care both in prevention and in the specific treatment. This study aimed to assess nurses' practice regarding closed wound care in the surgical unit, and evaluate the effect of a wound care protocol on nurses' practice in, and assess patients' wound healing pre- and post-applying wound care protocol in at two hospital Mansoura University and emergency hospitals. The study sample includes two groups first group, consisted of 55 nurses from the two hospitals, and second group consist 207 patients with closed wound. The sample of nurses received a suggested protocol regarding wound care. Data collection was accomplished utilizing three tools; Astructured interview for the nurses, wound care observational checklist, andBraden scale, wound assessment tool. Data was collected from both nurses and patients three times; one time before implementing the wound care protocol, and a second time after its implementation and third one after 12 week's follow up. The result of this study revealed a statistically significant improvement in nurses' practice regarding wound care after implementing wound care protocol with (P-value = 0.014 and 0.000 respectively). Additionally, wound degeneration decreased in post-protocol implementation and during follow-up. The wound regeneration significantly improved with (P-value = 0.000) after implementing the wound care protocol. Conclusion: the study concluded presence of an improvement in nurses' practice after implementing the wound care protocol. Also, the wound healing improved significantly. Recommendations: nurses' practice about surgical wound care and infection control in general needs to be updated, through in-service training protocol. The hospital should have policies regarding infection control measures, to be reviewed periodically.