BackGround: Adolescents self-care practices and hygienic care are a critical issues that determines the health status of them. This study aimed to assess the preventive self-care practices againsturinary tract infection (UTI) among secondary nursing students. A cross sectional descriptive design was adopted. All secondary nursing schools in Port-Said Governorate were the setting.  A total of 247of female nursing students were recruited suing simple random sampling technique. An interviewing Questionnaire sheet was used to assess students' self- care practices regarding prevention of UTI.The results revealed that 72.1% of girls had poor level of knowledge regarding anatomy and physiology of the urinary system and urinary tract infection. While, less than two-thirds of them had inadequate total practices.  The results showed that, strong positive correlation was detected between students' total knowledge score andtotal practice score. Conclusion: Majority of nursing students scored poor level of knowledge regarding UTIs and its preventive hygienic practices. Recommendations: more attention should be given to establish educational strategies towards improving female students' health awareness in this topic.