Background:Neonates in the NICUs are exposed to a number of painful procedures. Non-pharmacological treatment methods are effective to prevent and relieve the pain in newborns Aim: This study was aimed to improve nurses' knowledge regarding non-pharmacological management of pain in newborns in neonatal intensive care units. Subjects and Method: A quasi-experimental research design was utilized in this study. The subject of the study compromised all the nurses working in the NICUs in Port Said hospitals (N=40). The data were collected using a questionnaire sheet Conclusion: The study revealed that, after the educational program, more than half of the studied nurses had satisfactory knowledge regarding non- pharmacological pain management in newborn Recommendations: continuous training courses should be provided to nurses in order to keep their knowledge regarding non-pharmacological pain management up to date. Also, nurses should be encouraged to attend national and international conferences and workshops about non-pharmacological management for newborns.