Background: Infertility is a significantly health, social, and emotional problem in the society. Health related quality of life now is considered as an important outcome measure in many clinical settings. This study was aimed to assess quality of life among infertile couples at Mansoura University Hospital. A descriptive design was utilized in this study. A purposive sample including 80 infertile coupleswere participated in the current study.  It was carried out at El-Mansaura University Hospital. Tool A standardized tool i.e. FertiQol tool was administered to collect data and an informational booklet on “Coping with Infertility" were also given to the infertile couples were used for data collection. Results of this study revealed that, male partners had better quality of life than the infertile female partners in all the domains of quality of life i.e. emotional, mind/body, relational, social, environmental and tolerability domain and there was a significant difference in the quality of life of infertile male and female partners. The correlation between the various domains of infertile male and female partners showed that the emotional domain was positively correlated to other domains (except the environmental domain in female partners). Infertility had influenced all the domains of infertile couples but it had major impact on the emotional domain. There was a significant association between levels of Fertility Quality of life scores and age of couple. It was concluded that, the infertility affects all the domains of quality of life but it has major impact on the emotional aspect of the infertile couples. Therefore, the present study recommendedthat health professionals need to include assessment of psychological symptomatology to plan more efficient interventions to infertile patients.