Background: In Egypt more than 2500 mothers die every year from problems related to pregnancy and childbirth, leaving behind 200 motherless newborns. These deaths, can be avoided if mothers have proper information about access to pregnancy care. Maternal mortality in Egypt may be due to no or poor antenatal care, delay in seeking medical care, unwanted pregnancy, substandard care from team/ general practitioner and lack of blood bank. Aim of study determines guideline about antenatal care for pregnant women in Damietta City. Quasi–experimental design was utilized in this study. The study Sample The sample of this study consisted of 93 women from the total number 500 pregnant women attending all Maternal and Child Health Centers (MCH) at Damietta city according to inclusion criteria. The data were collected using two tools: The 1st tool: Interview Questionnaire to assess knowledge and practices of pregnant women about antenatal care, the 2nd tool was opinionnaire of pregnant women about booklet. The results of this study showed that, the total mean scores of knowledge of the studied sample in pre-test was (25.61±6.43), after implementation of the guideline, it raised to be (51.26±3.70). While the total mean score of practice in pre-test was (16.28±5.26), raised in post-test to be (29.65±2.43). Moreover a highly statistical significant differences were detected between pre and post test as regard knowledge and practice where p ≤ 0.001. The conclusion of this study was implementation of guideline about antenatal care improved pregnant women knowledge and practices about antenatal care. The study recommended thatBased on the present findings, It was recommended the following. Provision of posters & booklets about antenatal care within the Maternal and Child Health Centers (MCH) available to see by pregnant women. Further research about different methods for maternal care education.